Gqaleni N, Smith J E, Lacey J
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK.
Food Addit Contam. 1996 Aug-Sep;13(6):677-85. doi: 10.1080/02652039609374453.
The distribution of total aflatoxin (AFT) and cyclopiazonic acid (CPA) between conidia and mycelial matrix was studied in five isolates of Aspergillus flavus Link cultured on maize grain for 20 days at 30 degrees C. Total aflatoxin and CPA production differed between the isolates with Aspergillus flavus F2R4FP 1-5 producing the most AFT (conidia-0.245 microgram/g; mycelial matrix-83 micrograms/g) and CPA (conidia-0.091 microgram/g; mycelial matrix-37 micrograms/g). The production of AFT and CPA by this isolate was compared in liquid (malt-extract-yeast-extract-glucose) and solid substrates (yeast extract sucrose agar and maize) at 30 degrees C. The ratio of AFT:CPA in maize decreased from 16.6:1 to 1.6:1 between 7 and 21 days' incubation. In liquid culture and yeast extract sucrose agar, the ratios were reversed and ranged from 1:3 to 1:9 and 1:22 to 1:28, respectively, the greatest difference in both media occurring after 15 days.
研究了在30℃下于玉米粒上培养20天的五株黄曲霉Link分离株中，总黄曲霉毒素（AFT）和环匹阿尼酸（CPA）在分生孢子和菌丝体基质之间的分布情况。不同分离株的总黄曲霉毒素和CPA产量存在差异，其中黄曲霉F2R4FP 1-5产生的AFT最多（分生孢子-0.245微克/克；菌丝体基质-83微克/克），CPA也最多（分生孢子-0.091微克/克；菌丝体基质-37微克/克）。在30℃下，比较了该分离株在液体（麦芽提取物-酵母提取物-葡萄糖）和固体基质（酵母提取物蔗糖琼脂和玉米）中AFT和CPA的产生情况。在7至21天的培养期内，玉米中AFT:CPA的比例从16.6:1降至1.6:1。在液体培养和酵母提取物蔗糖琼脂中，比例相反，分别为1:3至1:9和1:22至1:28，两种培养基中最大差异均出现在15天后。
