溴化乙锭对培养的小鼠细胞中核糖体RNA产生的影响。
Effects of ethidium bromide on the production of ribosomal RNA in cultured mouse cells.
作者信息
Lange M, May P
出版信息
Nucleic Acids Res. 1979 Jun 25;6(8):2863-77. doi: 10.1093/nar/6.8.2863.
A treatment of primary mouse kidney cell cultures with 5 microM Ethidium Bromide (Eth Br) reduces the transcription of nuclear-coded genes and especially of ribosomal RNA genes. This effect was consistently observed when comparing drug-treated and control cells for (i), the incorporation of 3H uridine into total nuclear and B RNA polymerases as determined in isolated nuclei. It became more pronounced with exposure time; however, after removal of the drug, there was a progressive recovery of RNA synthesis culminating in the complete reversal of the drug effect. That this effect is probably not due only to the suppression of mitochondrial protein synthesis by the drug, is shown by a comparative study of the effects of chloramphenicol treatment. In addition, in the cytoplasm Eth Br depresses the labeling of 28 S rRNA more than that of 18 S whereas no abnormal accumulation of 28 S rRNA is observed in the nucleus. It is suggested that Eth Br may affect either the stability of the 28 S rRNA or its rate of formation from the 32 S precursor.
用5微摩尔溴化乙锭（Eth Br）处理原代小鼠肾细胞培养物会降低核编码基因的转录，尤其是核糖体RNA基因的转录。在比较药物处理细胞和对照细胞时，对于（i）在分离的细胞核中测定的3H尿苷掺入总核RNA和B RNA聚合酶的情况，始终观察到这种效应。随着暴露时间的延长，这种效应变得更加明显；然而，去除药物后，RNA合成逐渐恢复，最终药物效应完全逆转。氯霉素处理效果的比较研究表明，这种效应可能不仅仅是由于药物对线粒体蛋白质合成的抑制。此外，在细胞质中，Eth Br对28 S rRNA标记的抑制作用比对18 S rRNA的抑制作用更强，而在细胞核中未观察到28 S rRNA的异常积累。有人认为，Eth Br可能会影响28 S rRNA的稳定性或其从32 S前体形成的速率。
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