Allen M J, Lee J D, Lee C, Balhorn R
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA.
Mol Reprod Dev. 1996 Sep;45(1):87-92. doi: 10.1002/(SICI)1098-2795(199609)45:1<87::AID-MRD12>3.0.CO;2-U.
Volume measurements were performed on intact bull and mouse sperm heads and amembranous sperm nuclei, both in the fully hydrated (fluid cell) and dehydrated (air-dried on glass coverslips) states by atomic force microscopy (AFM). Data were obtained by analyzing a small population of cells/nuclei, as well as by performing repeated measurements on single cells imaged following the addition of increasing concentrations of propanol. Results show that the volume of fully hydrated, intact sperm heads and amembranous sperm chromatin particles are at least twice the volume of their air-dried counterparts. Dehydration occurs rapidly in air, and the reduction in volume of chromatin induced by water loss appears to be completely reversible. These studies demonstrate that both mouse and bull sperm chromatin are extensively hydrated in the native state, and are not as compact as previous studies have suggested.
通过原子力显微镜（AFM）对完整的公牛和小鼠精子头部以及无膜精子细胞核在完全水合（流体细胞）和脱水（在玻璃盖玻片上空气干燥）状态下进行体积测量。通过分析少量细胞/细胞核以及对添加递增浓度丙醇后成像的单个细胞进行重复测量来获得数据。结果表明，完全水合的完整精子头部和无膜精子染色质颗粒的体积至少是其空气干燥对应物体积的两倍。在空气中脱水迅速发生，并且由水分流失引起的染色质体积减少似乎是完全可逆的。这些研究表明，小鼠和公牛精子染色质在天然状态下都大量水合，并且不像以前的研究所表明的那样致密。
