National Resource for Imaging Mass Spectrometry, Division of Genetics, Harvard Medical School, Brigham and Women's Hospital, Cambridge, Massachusetts, United States of America.
PLoS One. 2012;7(8):e42267. doi: 10.1371/journal.pone.0042267. Epub 2012 Aug 27.
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.
在医学研究中，基因工程小鼠的应用越来越广泛，导致存储成本不断上升。海藻糖在部分干燥后保存在冰点以上的温度下，对保存小鼠精子的发育潜能有显著的有益作用。使用多同位素成像质谱法，我们能够对单个精子中的海藻糖进行成像和测量。我们首次提供的证据表明，海藻糖能够穿透哺乳动物细胞的核，从而允许细胞耐受干燥。这些结果对哺乳动物细胞的长期储存具有广泛的意义。
