Chan K M, Lam D T, Pong K, Widmer H R, Hefti F
Andru Geromtology Center, University of Southern California, Los Angeles 90089-0101, USA.
Neurochem Res. 1996 Jul;21(7):763-7. doi: 10.1007/BF02532298.
The aim of this study was to determine whether neurotrophin-4/5 (NT-4/5) treatment alters infarction volume following permanent focal cerebral ischemia in the rat. Permanent focal cerebral ischemia was produced in adult male rats by intraluminal occlusion of the right middle cerebral artery. NT-4/5 was administered intraventricularly one day before and immediately following occlusion. Rats were sacrificed at 1, 4 and 7 days after occlusion. NT-4/5 treatment reduced infarction volume by 34% when compared to control rats 1 day after occlusion. Infarction volume was unaltered by treatment 4 to 7 days after occlusion. Middle cerebral artery occlusion led to a significant reduction in levels of mRNAs coding for catalytic and truncated TrkB receptors. This expression was unaffected by NT-4/5 treatment.
本研究的目的是确定神经营养因子-4/5（NT-4/5）治疗是否会改变大鼠永久性局灶性脑缺血后的梗死体积。通过右大脑中动脉腔内闭塞法在成年雄性大鼠中制造永久性局灶性脑缺血。在闭塞前一天和闭塞后立即经脑室给予NT-4/5。在闭塞后1、4和7天处死大鼠。与对照组大鼠相比，闭塞后1天NT-4/5治疗使梗死体积减少了34%。闭塞后4至7天，治疗对梗死体积没有影响。大脑中动脉闭塞导致编码催化型和截短型TrkB受体的mRNA水平显著降低。这种表达不受NT-4/5治疗的影响。
