Müller K, Roth S, Fischer D C, Walther S, Dannhauer K H
Department of Orthodontics, Leipzig University.
J Orofac Orthop. 1996 Oct;57(5):310-21. doi: 10.1007/BF02197552.
Age-related changes in the relative proportion of high buoyant density proteoglycans of the soft tissue cover of the mandibular condyle of domestic pigs and the chemical composition of these proteoglycans were investigated by biochemical methods. The relative proportion of high buoyant density proteoglycans has been shown to decrease with age, with their chemical composition varying as follows: With age, the relative proportions of protein increased, galactosamine decreased, glucosamine was similar in newborns and juveniles, but clearly increased in adults, galactosamine plus glucosamine decreased. As measured by 1H-NMR-relaxation spectroscopic methods, the concentration of free tissue water of the soft tissue cover of the mandibular condyle decreased with age. Its mobility, however, was greater in newborns than in juveniles, but similar in juveniles and adults. The concentration of free tissue water, but not its mobility, correlated weakly with relative proportions of polyanionic side chains (e.g. chondroitin sulphate plus keratan sulphate chains) of the high buoyant density proteoglycans. By affecting the concentration of free tissue water and in turn of matrix solutes and cellular environments, the high buoyant density proteoglycans of the soft tissue cover of the mandibular condyle seem to be involved in regulation of age-related changes in the adaptive remodelling activity of the mandibular condyle.
采用生化方法研究了家猪下颌髁突软组织中高浮力密度蛋白聚糖相对比例的年龄相关变化以及这些蛋白聚糖的化学组成。结果表明，高浮力密度蛋白聚糖的相对比例随年龄增长而降低，其化学组成变化如下：随着年龄增长，蛋白质的相对比例增加，半乳糖胺减少，新生儿和幼年猪的葡萄糖胺含量相似，但成年猪明显增加，半乳糖胺加葡萄糖胺减少。通过1H-NMR弛豫光谱法测量，下颌髁突软组织中游离组织水的浓度随年龄增长而降低。然而，其流动性在新生儿中比在幼年猪中更大，但在幼年猪和成年猪中相似。游离组织水的浓度与其流动性不同，它与高浮力密度蛋白聚糖的聚阴离子侧链（如硫酸软骨素加硫酸角质素链）的相对比例弱相关。下颌髁突软组织中的高浮力密度蛋白聚糖似乎通过影响游离组织水的浓度，进而影响基质溶质和细胞环境，参与调节下颌髁突适应性重塑活动中的年龄相关变化。
