Murakami T, Ohtsuka A, Piao D X
Department of Anatomy, Okayama University School of Medicine, Japan.
Arch Histol Cytol. 1996 Aug;59(3):233-7. doi: 10.1679/aohc.59.233.
Many neurons in the human somatosensory cortex (Area 7 of Brodmann) possess an intensely negative-charged surface coat consisting of perineuronal sulfated proteoglycans which were stained with cationic iron colloid. This surface coat was stained doubly with cationic iron colloid and Golgi's silver nitrate. The result indicates that the perineuronal sulfated proteoglycans are identical with Golgi's reticular coating, whose existence and nature have previously been controversial. The result also suggests that Golgi's silver nitrate stains the core proteins of proteoglycans.
人类体感皮层(布罗德曼第7区)中的许多神经元都拥有一层带强负电荷的表面被膜,该被膜由神经周硫酸化蛋白聚糖组成,这些蛋白聚糖可用阳离子铁胶体染色。这种表面被膜用阳离子铁胶体和高尔基硝酸银进行双重染色。结果表明,神经周硫酸化蛋白聚糖与高尔基网状被膜相同,其存在和性质此前一直存在争议。该结果还表明,高尔基硝酸银可对蛋白聚糖的核心蛋白进行染色。