Kaca W, Mara M, Ocenaskova J
Institute of Microbiology and Immunology, University of Lódź, Banacha, Poland.
Arch Immunol Ther Exp (Warsz). 1996;44(1):39-44.
The ability of bacterial endotoxins, of different origin, to modify the level of mouse liver cytochrome P-450 was investigated. Endotoxins, (lipopolysaccharides, LPSs) were isolated from Proteus, Escherichia, Salmonella, Bacteroides and Coxiella strains. The most potent inhibitor of cytochrome P-450 activity was S. typhi 0101 LPS, which at a dose of 1 microgram/mouse reduced the cytochrome P-450 activity to 59%. E. coli O55:B5, S. typhimurium, P. mirabilis O3, and C. burnetii LPSs, at dose 10 micrograms/mouse, decrease cytochrome P-450 level from 56 to 69%. B. ovatus LPS significantly suppressed the expression of cytochrome P-450 only at the highest dose used-100 micrograms/mouse. The comparison of inhibitory activity of P. mirabilis complete, S and R types of LPSs indicate that lipid A portion of LPSs are sufficient to decrease the cytochrome P-450 level. However, the core oligosaccharide of LPS significantly enhance that inhibition. The isolated O-specific polysaccharide part of P. mirabilis O3 LPS did not decrease cytochrome P-450 level. The comparison of biological activity of Proteus LPSs, tested by chromogenic Limulus amebocyte lysate (LAL) test, demonstrated the enhancement effect of O-polysaccharide part of tested LPSs.
研究了不同来源的细菌内毒素对小鼠肝脏细胞色素P-450水平的影响。内毒素（脂多糖，LPS）从变形杆菌、大肠杆菌、沙门氏菌、拟杆菌和柯克斯氏体菌株中分离得到。细胞色素P-450活性最有效的抑制剂是伤寒沙门氏菌0101 LPS，剂量为1微克/小鼠时，细胞色素P-450活性降至59%。大肠杆菌O55:B5、鼠伤寒沙门氏菌、奇异变形杆菌O3和伯纳特柯克斯氏体LPS，剂量为10微克/小鼠时，细胞色素P-450水平从56%降至69%。卵形拟杆菌LPS仅在使用的最高剂量100微克/小鼠时显著抑制细胞色素P-450的表达。奇异变形杆菌完整型、S型和R型LPS抑制活性的比较表明，LPS的脂质A部分足以降低细胞色素P-450水平。然而，LPS的核心寡糖显著增强了这种抑制作用。奇异变形杆菌O3 LPS分离出的O-特异性多糖部分未降低细胞色素P-450水平。通过显色鲎试剂（LAL）试验测试的变形杆菌LPS的生物活性比较表明，受试LPS的O-多糖部分具有增强作用。
