Klink M, Sonn C H, Kaca W, Kim Y S
Microbiology and Virology Center, Polish Academy of Sciences, Lódź, Poland.
Arch Immunol Ther Exp (Warsz). 1999;47(3):195-201.
The biological activities were investigated of Proteus mirabilis lipopolysaccharides (LPSs) and their fragments, namely, the induction of nitric oxide (NO) and interleukin 1 (IL-1) synthesis by murine macrophages and the proliferation of murine spleen cells. The O-specific polysaccharide (F1), core oligosaccharide (F2) and lipid A were as effective as intact LPS in stimulating murine macrophages to produce NO. IL-1 synthesis was also induced by all studied types of endotoxins (S, Ra, Re) and partial structures, however F1, F2 and lipid A fractions required the presence of serum. In contrast to LPS, the O-specific polysaccharide, core oligosaccharide and lipid A were not able to induce the blast response of murine non-adherent splenocytes.
研究了奇异变形杆菌脂多糖（LPSs）及其片段的生物学活性，即小鼠巨噬细胞诱导一氧化氮（NO）和白细胞介素1（IL-1）合成以及小鼠脾细胞增殖的情况。O-特异性多糖（F1）、核心寡糖（F2）和脂质A在刺激小鼠巨噬细胞产生NO方面与完整LPS一样有效。所有研究类型的内毒素（S、Ra、Re）及其部分结构也能诱导IL-1合成，然而F1、F2和脂质A组分需要血清存在。与LPS不同，O-特异性多糖、核心寡糖和脂质A不能诱导小鼠非贴壁脾细胞的母细胞反应。
