Kalus P, Senitz D
Department of Psychiatry, University of Würzburg, Germany.
Brain Res. 1996 Aug 5;729(1):45-54.
The calcium-binding protein parvalbumin (PV) is a marker for a certain subset of GABAergic cortical interneurons. In the present study, indirect immunocytochemistry with an antibody against PV was performed on serial sections of human anterior cingulate cortex (Brodmann's area 24), an important relay centre of the limbic system. PV-positive structures are distributed in a layer- and cell type-specific manner. Based on morphological features and laminar distribution pattern, PV-immunoreactive interneurons are subdivided into eight different classes. PV immunoreactivity within the neuropil comprises dendritic and axonal processes. Area 24 contains two densely immunolabelled neuropil bands in layers III and Vb. Axon cartridges are preferably located in layers V and VI. The results provide a "PV immunoarchitecture' as a basis for further studies of PV immunoreactivity under pathological conditions. PV is assumed to play a role in maintaining calcium homeostasis in nerve cells, and to modulate neuronal excitability and resistance to biochemical damage. On the other hand, PV immunoreactivity has recently been shown to undergo characteristic changes during different stages of brain maturation. Therefore, examination of PV-positive structures will provide new insights into cortical circuitry in neurodegenerative as well as neurodevelopmental disorders.
钙结合蛋白小白蛋白（PV）是一类γ-氨基丁酸能皮质中间神经元的标志物。在本研究中，我们使用抗PV抗体对人类前扣带回皮质（布罗德曼24区）的连续切片进行了间接免疫细胞化学实验，该区域是边缘系统的一个重要中继中心。PV阳性结构呈层特异性和细胞类型特异性分布。基于形态学特征和层状分布模式，PV免疫反应性中间神经元可细分为八个不同类别。神经毡内的PV免疫反应性包括树突和轴突。24区在III层和Vb层包含两条免疫标记密集的神经毡带。轴突小体主要位于V层和VI层。这些结果提供了一种“PV免疫结构”，作为进一步研究病理条件下PV免疫反应性的基础。PV被认为在维持神经细胞内钙稳态、调节神经元兴奋性以及抵抗生化损伤方面发挥作用。另一方面，最近研究表明，PV免疫反应性在大脑成熟的不同阶段会发生特征性变化。因此，对PV阳性结构的研究将为神经退行性疾病和神经发育障碍中的皮质回路提供新的见解。
