Ang H H, Lam C K, Wah M J
School of Pharmaceutical Sciences, University of Science Malaysia, Minden, Penang, Malaysia.
Chemotherapy. 1996 Sep-Oct;42(5):318-23. doi: 10.1159/000239461.
Six clones were derived from each Plasmodium falciparum isolate obtained from Malaysia, Africa and Thailand and were characterized against type II antifolate drugs, cycloguanil and pyrimethamine using the modified in vitro microtechnique. Results showed that these isolates were of a heterogeneous population, with 50% inhibitory concentrations of Gombak A clones at 0.0151-0.1450 and 0.0068-0.1158 microM, Gambian clones at 0.0056-0.1792 and 0.0004-0.0068 microM and TGR clones at 0.0103-0.0703 and 0.0776-0.3205 microM against cycloguanil and pyrimethamine, respectively. All clones displayed similar susceptibilities as their parent isolates except A/D3, A/D5, A/G4 and A/H7 clones which were sensitive to cycloguanil at 0.0735, 0.0151, 0.0540 and 0.0254 microM but Gm/B2 clone was resistant at 0.1792 microM, respectively. However, A/D3, TGR/B4, TGR/B7, TGR/C4, TGR/C7 and TGR/H2 clones were resistant to pyrimethamine at 0.1158, 0.1070, 0.1632, 0.1580, 0.2409 and 0.3205 microM, respectively. Further results indicated that they were pure clones compared to their parent isolates as their drug susceptibility studies were statistically different (p < 0.05).
从马来西亚、非洲和泰国获得的每一株恶性疟原虫分离株都衍生出6个克隆，并使用改良的体外微技术针对II型抗叶酸药物环氯胍和乙胺嘧啶进行特性分析。结果显示，这些分离株属于异质群体，Gombak A克隆对环氯胍和乙胺嘧啶的50%抑制浓度分别为0.0151 - 0.1450和0.0068 - 0.1158微摩尔，冈比亚克隆分别为0.0056 - 0.1792和0.0004 - 0.0068微摩尔，TGR克隆分别为0.0103 - 0.0703和0.0776 - 0.3205微摩尔。除了A/D3、A/D5、A/G4和A/H7克隆对环氯胍敏感，其浓度分别为0.0735、0.0151、0.0540和0.0254微摩尔，而Gm/B2克隆在0.1792微摩尔时耐药外，所有克隆与其亲本分离株表现出相似的敏感性。然而，A/D3、TGR/B4、TGR/B7、TGR/C4、TGR/C7和TGR/H2克隆对乙胺嘧啶耐药，浓度分别为0.1158、0.1070、0.1632、0.1580、0.2409和0.3205微摩尔。进一步的结果表明，与它们的亲本分离株相比，它们是纯克隆，因为它们的药物敏感性研究在统计学上有差异（p < 0.05）。
