Clavel A, Arnal A, Sánchez E, Varea M, Quílez J, Ramírez I, Castillo F J
Servicio de Microbiología y Parasitología, Hospital Clínico Universitario, Zaragoza, Spain.
Int J Parasitol. 1996 Jun;26(6):671-2. doi: 10.1016/0020-7519(96)00057-4.
A comparison between the standard formalin-ethyl acetate (FEA) stool concentration procedure (centrifugation at 400 g for 2 min) and a modification proposed to increase the recovery of Cryptosporidium oocysts (500 g for 10 min) was performed on 73 formalin-preserved stool specimens known to be positive for Cryptosporidium. False-negative results were obtained with both methods, but the increase in centrifugation time and force resulted in the detection of a higher number of oocysts and positive samples. The modified procedure had a sensitivity significantly higher than the standard method (99% compared to 86%, P = 0.0045).
对73份已知隐孢子虫呈阳性的经福尔马林保存的粪便标本，进行了标准福尔马林 - 乙酸乙酯（FEA）粪便浓缩程序（400 g离心2分钟）与为提高隐孢子虫卵囊回收率而提出的改良程序（500 g离心10分钟）之间的比较。两种方法均出现了假阴性结果，但离心时间和力度的增加导致检测到的卵囊数量和阳性样本增多。改良程序的灵敏度显著高于标准方法（分别为99%和86%，P = 0.0045）。
