Chiodera P, Volpi R, Capretti L, Caffarri G, Magotti M G, Coiro V
Department of Internal Medicine, School of Medicine, University of Parma, Italy.
Neuroendocrinology. 1996 Sep;64(3):242-6. doi: 10.1159/000127124.
The possible stimulatory effects of an intravenous infusion of increasing amounts of pituitary adenylate cyclase-activating polypeptide (PACAP) on anterior pituitary hormone secretions were evaluated in humans. Successively increasing doses of PACAP-38 (2, 4 and 8 pmol.kg-1.min-1; each dose for 20 min) were infused i.v. in 7 normal male subjects. On a different occasion, the same subjects were tested with vasoactive intestinal peptide (VIP; 4 pmol.kg-1.min-1 for 60 min). Circulating GH, ACTH, PRL, TSH and gonadotropin concentrations were measured before PACAP infusion and every 20 min, just before increasing the infusion dose of PACAP. Blood samples were taken before and every 15 min after the beginning of VIP administration. Serum levels of GH, TSH and gonadotropins did not change during PACAP or VIP infusion. Circulating ACTH and PRL concentrations were not modified by the infusion of the lowest dose of PACAP, whereas they were significantly increased in a dose-response fashion when higher amounts of PACAP were given. PRL, but not ACTH levels were significantly increased by VIP infusion. These data show for the first time in humans that ACTH and PRL secretions from the anterior pituitary gland are stimulated by the systemic administration of PACAP. In addition, since VIP stimulated only PRL secretion, PACAP-induced ACTH release appears to be mediated by specific receptors.
在人类中评估了静脉输注递增剂量的垂体腺苷酸环化酶激活多肽（PACAP）对垂体前叶激素分泌的可能刺激作用。对7名正常男性受试者静脉输注依次递增剂量的PACAP-38（2、4和8 pmol·kg⁻¹·min⁻¹；每个剂量持续20分钟）。在另一个时间点，对相同的受试者用血管活性肠肽（VIP；4 pmol·kg⁻¹·min⁻¹，持续60分钟）进行测试。在输注PACAP之前以及在每次增加PACAP输注剂量之前每20分钟测量循环中的生长激素（GH）、促肾上腺皮质激素（ACTH）、催乳素（PRL）、促甲状腺激素（TSH）和促性腺激素浓度。在开始给予VIP之前和之后每15分钟采集血样。在输注PACAP或VIP期间，血清GH、TSH和促性腺激素水平没有变化。输注最低剂量的PACAP时，循环中的ACTH和PRL浓度没有改变，而给予更高剂量的PACAP时，它们以剂量反应方式显著增加。VIP输注显著增加了PRL水平，但没有增加ACTH水平。这些数据首次在人类中表明，全身给予PACAP可刺激垂体前叶分泌ACTH和PRL。此外，由于VIP仅刺激PRL分泌，PACAP诱导的ACTH释放似乎是由特定受体介导的。
