Hophy M, Harel S, Yavin E
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Reprod Fertil Dev. 1996;8(5):895-901. doi: 10.1071/rd9960895.
An experimental model was devised in the pregnant rat to study by a combined high pressure liquid chromatography and radioimmunoassay technique the accumulation of prostanoids (PNs) in the urine after transient-complete or permanent-partial interruption of the maternal-fetal blood flow. After 8 min of complete restriction of the blood flow in the pregnant rat at 18 days of gestation, the urinary concentration of 6-keto-prostaglandin F1 alpha (6k-PGF1 alpha, the stable prostacyclin metabolite) increased from 4.97 +/- 1.27 ng mg-1 creatinine to 8.09 +/- 2.47 ng mg-1 creatinine and 13.02 +/- 4.5 ng mg-1 creatinine after the second and third post-operative day respectively. The urinary concentration of the 2,3-dinor derivative of prostacyclin reached 12.35 +/- 5.44 ng mg-1 creatinine after the second post-operative day and was reduced to 4.71 +/- 1.94 ng mg-1 creatinine after the third post-operative day. The concentration of thromboxane B2 (TxB2, the stable thromboxane A2 metabolite) increased approximately 7-fold and 13-fold over that of the control after the second and third post-operative day respectively. The urinary concentration of the 2,3-dinor derivative of TxB2 (d-TxB2) increased from about 1.42 +/- 0.3 ng mg-1 creatinine to 4.49 +/- 0.9 ng mg-1 creatinine and 7.76 +/- 2.63 ng mg-1 creatinine under the same experimental conditions. Increases in the urinary concentrations of 6k-PGF1 alpha and d-TxB2 to 94 +/- 27.76 ng mg-1 creatinine and 12.05 +/- 2.26 ng mg-1 creatinine, respectively, were observed on the second post-operative day, after the restriction time was increased to 30 min. Permanent-partial occlusion of the maternal fetal circulation resulted in excretion of PNs in the urine to similar levels produced after transient-complete restriction. High concentrations of prostacyclin (range, 0.8 ng min-1 mg-1 wet weight) were produced in vitro by uterine preparations from restricted animals after the second post-operative day. Placenta preparations from restricted animals generally exhibited a lower ability to synthesize PNS (up to 0.006 ng min-1 mg-1 wet weight) compared with uterine tissue but produced more thromboxane than their sham counterparts. The data suggest that the uterus constitutes the main source for urinary PN excretion following short episodes of maternal-fetal blood flow interruption.
设计了一种孕鼠实验模型，通过高压液相色谱和放射免疫分析技术相结合的方法，研究母体-胎儿血流短暂完全阻断或永久性部分阻断后尿液中前列腺素（PNs）的蓄积情况。在妊娠18天的孕鼠中，完全阻断血流8分钟后，6-酮-前列腺素F1α（6k-PGF1α，稳定的前列环素代谢产物）的尿浓度从4.97±1.27 ng mg-1肌酐分别在术后第二天和第三天增加到8.09±2.47 ng mg-1肌酐和13.02±4.5 ng mg-1肌酐。前列环素的2,3-二去甲衍生物的尿浓度在术后第二天达到12.35±5.44 ng mg-1肌酐，在术后第三天降至4.71±1.94 ng mg-1肌酐。血栓素B2（TxB2，稳定的血栓素A2代谢产物）的浓度在术后第二天和第三天分别比对照组增加了约7倍和13倍。在相同实验条件下，TxB2的2,3-二去甲衍生物（d-TxB2）的尿浓度从约1.42±0.3 ng mg-1肌酐增加到4.49±0.9 ng mg-1肌酐和7.76±2.63 ng mg-1肌酐。在术后第二天，当阻断时间增加到30分钟后，观察到6k-PGF1α和d-TxB2的尿浓度分别增加到94±27.76 ng mg-1肌酐和12.05±2.26 ng mg-1肌酐。母体-胎儿循环的永久性部分闭塞导致尿液中PNs的排泄水平与短暂完全阻断后产生的水平相似。术后第二天，来自血流受限动物的子宫制剂在体外产生了高浓度的前列环素（范围为0.8 ng min-1 mg-1湿重）。与子宫组织相比，来自血流受限动物的胎盘制剂通常合成PNS的能力较低（高达0.006 ng min-1 mg-1湿重），但产生的血栓素比假手术组更多。数据表明，子宫是母体-胎儿血流中断短时间后尿液中PN排泄的主要来源。
