Uehara F, Ohba N, Sameshima M, Yanagita T, Iwakiri N, Ozawa M, Miyagi T
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Jpn J Ophthalmol. 1996;40(2):187-91.
The binding sites of the antibody to cytosolic sialidase on the rat and monkey photoreceptor cells were examined immunohistochemically using the avidin-biotinylated peroxidase method. In the rat photoreceptor cells, the antibody bound diffusely to the inner segment and the outer nuclear layers which are composed chiefly of rod cells. In the monkey photoreceptor cells, the antibody bound to the rod inner segments which were clearly distinct, morphologically, from the cone inner segments. The antibody also bound to the rod cell bodies in the outer nuclear layer. These binding patterns show that the antibody bound preferentially to rod photoreceptor cells. This observation is consistent with previous lectin histochemical findings that sialoglycans are preferentially present on the surfaces of rod photoreceptors, and in the rod-associated interphotoreceptor matrix. Sialidase in rod inner segments may function by balancing with sialyltransferase, also preferentially expressed in rod inner segments, to form sialyl residues on the termini of sugar chains in the rod-associated sialoglycoconjugates.
使用抗生物素蛋白-生物素化过氧化物酶法，通过免疫组织化学检查了抗体与大鼠和猴感光细胞中胞质唾液酸酶的结合位点。在大鼠感光细胞中，抗体广泛结合至主要由视杆细胞组成的内段和外核层。在猴感光细胞中，抗体结合至视杆内段，其在形态上与视锥内段明显不同。抗体还结合至外核层中的视杆细胞体。这些结合模式表明，抗体优先结合视杆感光细胞。该观察结果与先前的凝集素组织化学研究结果一致，即唾液酸聚糖优先存在于视杆感光细胞表面以及与视杆相关的光感受器间基质中。视杆内段中的唾液酸酶可能通过与同样优先在视杆内段表达的唾液酸转移酶平衡，在与视杆相关的唾液酸糖缀合物的糖链末端形成唾液酸残基来发挥作用。
