Liu X, Seno K, Nishizawa Y, Hayashi F, Yamazaki A, Matsumoto H, Wakabayashi T, Usukura J
Department of Anatomy, Nagoya University, School of Medicine, Japan.
Exp Eye Res. 1994 Dec;59(6):761-8. doi: 10.1006/exer.1994.1162.
Immuno-imaging with confocal and electron microscopy revealed the localization of retinal guanylate cyclase (RetGC) in human and monkey retinas. Using an antibody against a peptide derived from human RetGC, RetGC was found predominantly in the photoreceptor layer in these retinas, although a small amount of RetGC was detected in various other retinal cells. In particular, the cone outer segments were more densely labeled with the antibody than the rod outer segments. The RetGC in outer segments was localized exclusively in the membrane-rich domains, and appeared to be associated with the marginal region of the disk membrane and/or the plasma membrane. The connecting cilium and its cytoplasmic extension never showed immunoreactivity with the antibody. The localization of RetGC in photoreceptor cells is discussed from the viewpoint of mechanisms for the recovery of photoreceptors to the dark level.
共聚焦显微镜和电子显微镜免疫成像揭示了视网膜鸟苷酸环化酶（RetGC）在人类和猴子视网膜中的定位。使用针对源自人类RetGC的肽段的抗体，发现RetGC主要存在于这些视网膜的光感受器层中，尽管在其他各种视网膜细胞中也检测到少量的RetGC。特别是，与视杆细胞外段相比，视锥细胞外段被抗体标记得更密集。外段中的RetGC仅定位在富含膜的区域，并且似乎与盘膜的边缘区域和/或质膜相关。连接纤毛及其细胞质延伸部分从未显示出与该抗体的免疫反应性。从光感受器恢复到暗水平的机制角度讨论了RetGC在光感受器细胞中的定位。
