Scharfman A, Van Brussel E, Houdret N, Lamblin G, Roussel P
Unité INSERM No. 377, Lille, France.
Am J Respir Crit Care Med. 1996 Oct;154(4 Pt 2):S163-9. doi: 10.1164/ajrccm/154.4_Pt_2.S163.
Pseudomonas aeruginosa binds to different glycoconjugates in vitro. As six other bacteria, it binds to several glycolipids, mainly asialo GM1 and asialo GM2. Asialo GM1 has been reported to exist at the surface of cystic fibrosis cells. The binding of P. aeruginosa to asialo GM1 involves the pili, especially the C-terminal part of pilin that recognizes the GaINAc(beta 1,4) Gal sequence of asialo GM1.P. aeruginosa may also bind to sialylated membrane-bound glycoproteins. Human salivary and respiratory mucins are also recognized by P. aeruginosa. Mucins represent the main components of mucus. The peptide part (apomucin) of this broad family of secreted glycoproteins is encoded by several mucin genes. The apomucins are covered by a large number of carbohydrate chains that can be remarkably different and represent a mosaic of sites for attachment of microorganisms. The binding of P. aeruginosa to mucins involves outer membrane proteins and mucin carbohydrate chains that are structurally different from the carbohydrate recognized by pillin. Airway and salivary mucins secreted by patients suffering from cystic fibrosis (CF) show alterations in their carbohydrate moiety. The increased sulfation of airway mucins seems to correspond to a primary defect. Other abnormalities such as increased sialylation or fucosylation have also been detected. The binding of P. aeruginosa to airway or salivary mucins is increased in CF. However, the precise link between the carbohydrate alterations and the increased binding of P. aeruginosa to CF mucins remains to be elucidated.
铜绿假单胞菌在体外可与不同的糖缀合物结合。与其他六种细菌一样，它能与多种糖脂结合，主要是去唾液酸GM1和去唾液酸GM2。据报道，去唾液酸GM1存在于囊性纤维化细胞表面。铜绿假单胞菌与去唾液酸GM1的结合涉及菌毛，尤其是菌毛蛋白的C端部分，它能识别去唾液酸GM1的GaINAc(β1,4)Gal序列。铜绿假单胞菌也可能与唾液酸化的膜结合糖蛋白结合。人唾液和呼吸道黏蛋白也能被铜绿假单胞菌识别。黏蛋白是黏液的主要成分。这类分泌型糖蛋白大家族的肽部分（脱辅基黏蛋白）由多个黏蛋白基因编码。脱辅基黏蛋白被大量碳水化合物链覆盖，这些碳水化合物链可能有显著差异，代表了微生物附着的位点镶嵌图。铜绿假单胞菌与黏蛋白的结合涉及外膜蛋白和黏蛋白碳水化合物链，其结构与菌毛蛋白识别的碳水化合物不同。囊性纤维化（CF）患者分泌的气道和唾液黏蛋白在其碳水化合物部分显示出改变。气道黏蛋白硫酸化增加似乎对应于一个原发性缺陷。还检测到了其他异常，如唾液酸化或岩藻糖基化增加。在CF中，铜绿假单胞菌与气道或唾液黏蛋白的结合增加。然而，碳水化合物改变与铜绿假单胞菌与CF黏蛋白结合增加之间的确切联系仍有待阐明。
