Do Thanh X, Massicot F, Do B, Breget R, Nivet V, Durand D, Warnet J M, Claude J R, Clot J P
Laboratoire de Biochimie/Physiologie, UFR de Médecine/Pharmacie, Université d'Angers, France.
Acta Physiol Scand. 1996 Sep;158(1):107-11. doi: 10.1046/j.1365-201X.1996.481282000.x.
The effects of 1 alpha,25-dihydroxyvitamin D3 (1,25 (OH)2D3) and human growth hormone (hGH) on the activity of alkaline phosphatase (ALP) were evaluated in pig kidney LLC-PK1 cultured cells. 1,25(OH)2D3 (10(-9)-10(-6) mol L-1) and hGH (10(-7) mol L-1) increased ALP activity in these cells while no hormonal effect was detected on two other enzyme activities: gamma-glutamyl transferase and acid phosphatase. ALP activity was maximally increased after 4.5-6 h incubation with both hormones. The hormonal induction of ALP activity was prevented by a pretreatment of cells with actinomycin D. Thus, 1,25 (OH)2D3 and may be hGH could stimulate ALP activity via a transcription of some gene.
