Kondo K, Makita T
Department of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Japan.
J Vet Med Sci. 1996 Aug;58(8):743-8. doi: 10.1292/jvms.58.743.
Although many reports are available to indicate the increase in number and size of peroxisomes by oral administration of hypolipidemic reagents such as clofibrate and bezafibrate, the decrease in number and size of proliferated peroxisomes even during administration of these chemicals has not been well documented. The present study was aimed to detect when the decrease in number and size of peroxisomes would occur. In the present study, ultrastructural changes in hepatocytes of male Wistar rats during 3 to 90 days of oral administration of bezafibrate were analyzed by morphometric and cytochemical methods. By 3-day administration, the weight of the liver and the number and size of peroxisomes were significantly increased. These parameters began to decline from the time 14 days of administration when the weight of the liver and the size of peroxisomes attained the peak. Heterogeneous catalase staining was most remarkable by 7-day administration. Mitochondria were another target cell organelle by bezafibrate. The total area of mitochondria was decreased by 3-day administration. However, by subsequent 7 to 90-day administration, it recovered almost the level of controls. By 90-day administration, many mitochondria had expanded cristae which contained filamentous substances.
尽管有许多报告表明，通过口服氯贝丁酯和苯扎贝特等降血脂试剂可使过氧化物酶体的数量增加和体积增大，但即使在使用这些化学物质期间，增殖的过氧化物酶体数量减少和体积变小的情况却没有得到充分的记录。本研究旨在检测过氧化物酶体数量减少和体积变小会在何时发生。在本研究中，通过形态计量学和细胞化学方法分析了雄性Wistar大鼠口服苯扎贝特3至90天期间肝细胞的超微结构变化。给药3天时，肝脏重量以及过氧化物酶体的数量和体积显著增加。从给药14天开始，当肝脏重量和过氧化物酶体体积达到峰值时，这些参数开始下降。给药7天时，过氧化氢酶的异质性染色最为明显。线粒体是苯扎贝特作用的另一个靶细胞器。给药3天时线粒体的总面积减少。然而，在随后的7至90天给药期间，其几乎恢复到对照水平。给药90天时，许多线粒体的嵴扩张，其中含有丝状物质。
