Maeda T, Kajiyama K, Adachi E, Takenaka K, Sugimachi K, Tsuneyoshi M
Department of Pathology II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Mod Pathol. 1996 Sep;9(9):901-9.
We performed immunohistochemical studies on 90 surgically resected liver tumors, including 30 tumors each from hepatocellular carcinoma (HCC), cholangiocarcinoma (CC), and metastatic colorectal adenocarcinoma (MCA), using monoclonal antibodies against cytokeratin (CK) 7, CK 19, and CK 20 to examine the differences in the CK expressions in primary and metastatic carcinomas of the liver. We also investigated the usefulness of such expression in the differential diagnosis in addition to existing markers such as alpha-fetoprotein, carcinoembryonic antigen, and carbohydrate antigen 19-9. For CK 7, all except for one (97%) of the CCs were diffusely positive, whereas only two (7%) HCCs and one (3%) MCAs were diffusely positive. For CK 19, 23 (77%) CCs and 19 (64%) MCAs were diffusely positive, whereas no HCCs were positive. For CK 20, 22 (74%) MCAs were diffusely positive, whereas no HCC and three (10%) CCs were diffusely positive. The findings concerning the expression of immunohistochemical CK are therefore considered to be useful in addition to the diagnostic criteria when making a differential diagnosis of primary and metastatic carcinomas of the liver.
我们使用抗细胞角蛋白（CK）7、CK 19和CK 20的单克隆抗体，对90例手术切除的肝肿瘤进行了免疫组织化学研究，其中包括30例肝细胞癌（HCC）、30例胆管癌（CC）和30例转移性结直肠癌（MCA），以研究原发性和转移性肝癌中CK表达的差异。除了现有的标志物，如甲胎蛋白、癌胚抗原和糖类抗原19-9外，我们还研究了这种表达在鉴别诊断中的实用性。对于CK 7，除1例（97%）胆管癌外，其余均呈弥漫性阳性，而只有2例（7%）肝细胞癌和1例（3%）转移性结直肠癌呈弥漫性阳性。对于CK 19，23例（77%）胆管癌和19例（64%）转移性结直肠癌呈弥漫性阳性，而肝细胞癌均为阴性。对于CK 20，22例（74%）转移性结直肠癌呈弥漫性阳性，而肝细胞癌均为阴性，3例（10%）胆管癌呈弥漫性阳性。因此，免疫组织化学CK表达的研究结果在鉴别原发性和转移性肝癌时，除诊断标准外，被认为是有用的。
