Zschauer A O, Davis E B, Anderson D R
Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Fla., USA.
Ophthalmologica. 1996;210(5):276-9. doi: 10.1159/000310725.
To characterize the relaxation of pericytes induced by beta-adrenergic stimulation, changes in the contractile tone of pericytes were quantified as a change in the wrinkling of an elastic silicone surface on which they were cultured. Isoproterenol produced relaxation of pericytes in a dose-dependent manner over a range of 5 nM to 1 microM. Low concentrations of the nonselective beta-blockers propanolol and timolol blocked the relaxation produced by isoproterenol. The specific beta 2-adrenergic component of isoproterenol-induced relaxation was shown by blockage with bromoacetyl alprenolol menthane. In contrast, atenolol and betaxolol, as relatively selective beta 1-adrenergic blockers, had no effect on the isoproterenol-induced relaxation.
为了表征β-肾上腺素能刺激诱导的周细胞舒张，将周细胞收缩张力的变化量化为其培养的弹性硅酮表面皱纹的变化。异丙肾上腺素在5 nM至1 μM范围内以剂量依赖性方式使周细胞舒张。低浓度的非选择性β-阻滞剂普萘洛尔和噻吗洛尔可阻断异丙肾上腺素诱导的舒张。溴乙酰阿普洛尔薄荷烷阻断表明异丙肾上腺素诱导舒张中有特定的β2-肾上腺素能成分。相比之下，作为相对选择性β1-肾上腺素能阻滞剂的阿替洛尔和倍他洛尔对异丙肾上腺素诱导的舒张没有影响。
