Bock C T, Schwinn S, Schröder C H, Velhagen I, Zentgraf H
German Cancer Research Centre, Applied Tumor Virology, Heidelberg, FRG.
Virus Genes. 1996;12(1):53-63. doi: 10.1007/BF00370001.
An early step in the replication of the hepatitis B virus (HBV) genome is the transport of the viral DNA into the nucleus of the infected cell. So far only little is known about the events and mechanisms at the nuclear membrane required for entry of the viral genome into the nucleus. Using a hepatoblastoma cell line that constitutively produces hepatitis B virions and in so doing displays intracellular viral amplification, we showed that nonparticulated HBV core protein is associated with nuclear membrane pore complexes. Additionally, viral DNA has been detected firmly attached to the nuclear membrane. Small amounts of viral core protein, as well as viral DNA, were detectable within the cell nucleus. However, core particles could not be shown at the nuclear membrane or within the nuclei of these cells. Our observations on localization of HBV DNA and core protein at the nuclear membrane thus provide a suggestion for further examinations of the transfer of the viral genome from the cytoplasm into the nucleus of the infected cell.
乙型肝炎病毒（HBV）基因组复制的早期步骤之一是病毒DNA转运至受感染细胞的细胞核。到目前为止，对于病毒基因组进入细胞核所需的核膜相关事件和机制了解甚少。我们使用一种持续产生乙型肝炎病毒粒子并因此显示细胞内病毒扩增的肝癌细胞系，发现非颗粒状的HBV核心蛋白与核膜孔复合体相关。此外，已检测到病毒DNA牢固附着于核膜。在细胞核内可检测到少量病毒核心蛋白以及病毒DNA。然而，在这些细胞的核膜处或细胞核内未发现核心颗粒。我们对HBV DNA和核心蛋白在核膜处定位的观察结果，为进一步研究病毒基因组从细胞质转移至受感染细胞的细胞核提供了线索。
