Guidotti L G, Martinez V, Loh Y T, Rogler C E, Chisari F V
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037.
J Virol. 1994 Sep;68(9):5469-75. doi: 10.1128/JVI.68.9.5469-5475.1994.
Transgenic mice that express the hepatitis B virus core protein were used to examine factors that influence the intracellular localization of nucleocapsid particles in the primary hepatocyte in vivo. In this model, viral nucleocapsid particles are strictly localized to the nucleus of the hepatocyte except when the nuclear membrane dissolves during cell division, at which time they enter the cytoplasm. The cytoplasmic nucleocapsid particles do not reenter the nucleus, however, when the nuclear membrane re-forms after cell division. The data support the notion that nucleocapsid particles can form de novo within the nucleus, and they suggest that performed nucleocapsid particles cannot be transported across the intact nuclear membrane in either direction. The results imply that nucleocapsid disassembly is probably required for entry of the hepadnaviral genome into the nucleus, and they question the role of the intranuclear viral nucleocapsid particle during the viral life cycle.
利用表达乙肝病毒核心蛋白的转基因小鼠来研究影响体内原代肝细胞中核衣壳颗粒细胞内定位的因素。在这个模型中，病毒核衣壳颗粒严格定位于肝细胞的细胞核，除非在细胞分裂期间核膜溶解，此时它们进入细胞质。然而，当细胞分裂后核膜重新形成时，细胞质中的核衣壳颗粒不会重新进入细胞核。这些数据支持核衣壳颗粒可在细胞核内重新形成的观点，并且表明已形成的核衣壳颗粒不能双向穿过完整的核膜。结果表明，核衣壳的拆解可能是嗜肝DNA病毒基因组进入细胞核所必需的，并且对核内病毒核衣壳颗粒在病毒生命周期中的作用提出了质疑。
