Fidler I J, Kripke M L
Science. 1977 Aug 26;197(4306):893-5. doi: 10.1126/science.887927.
Clones derived in vitro from a parent culture of murine malignant melanoma cells varied greatly in their ability to produce metastatic colonies in the lungs upon intravenous inoculation into syngeneic mice. This suggests that the parent tumor is heterogeneous and that highly metastatic tumor cell variants preexist in the parental population.
从鼠恶性黑色素瘤细胞的亲代培养物中体外衍生出的克隆，在静脉接种到同基因小鼠体内后，其在肺部产生转移性菌落的能力差异很大。这表明亲代肿瘤是异质性的，并且高转移性肿瘤细胞变体在亲代群体中预先存在。
