Bondy S C, Guo S X, Adams J D
Department of Community and Environmental Medicine, Irvine Occupational Health Center, University of California 92717-1825, USA.
Alcohol Alcohol. 1996 Jul;31(4):403-10. doi: 10.1093/oxfordjournals.alcalc.a008170.
Rats were given a 200 mg/kg body weight daily dose of alpha-tocopherol by i.p. injection for 15 days. This resulted in elevated levels of glutathione in both liver and brain, and in a reduced hepatic rate of generation of reactive oxygen species. The depression of hepatic and cerebral glutathione levels in ethanol-consuming rats was prevented by simultaneous treatment with alpha-tocopherol. Other putative indices of hepatic pro-oxidant events, namely levels of mixed function oxidase and proteolytic activity, were elevated by alpha-tocopherol both in the presence and absence of ethanol. In addition, levels of enzymes especially susceptible to oxidative degradation, glutamine synthetase and creatine kinase, were depressed in the liver following treatment with ethanol or alpha-tocopherol. Parameters rapidly responsive to oxidative changes revealed the antioxidant property of alpha-tocopherol, while protein-based indices reflecting more extended events suggested a pro-oxidant effect of this vitamin. Results suggest that high levels of alpha-tocopherol can simultaneously lead to a more reduced intracellular environment and yet to localized evidence of enhanced oxidative events.
通过腹腔注射，给大鼠每日注射200毫克/千克体重的α-生育酚，持续15天。这导致肝脏和大脑中的谷胱甘肽水平升高，并降低了肝脏中活性氧的生成速率。同时用α-生育酚治疗可预防饮酒大鼠肝脏和大脑中谷胱甘肽水平的降低。无论有无乙醇，α-生育酚都会提高肝脏促氧化事件的其他假定指标，即混合功能氧化酶水平和蛋白水解活性。此外，在用乙醇或α-生育酚治疗后，肝脏中对氧化降解特别敏感的酶，如谷氨酰胺合成酶和肌酸激酶的水平会降低。对氧化变化迅速做出反应的参数揭示了α-生育酚的抗氧化特性，而反映更广泛事件的基于蛋白质的指标表明该维生素具有促氧化作用。结果表明，高水平的α-生育酚可同时导致细胞内环境更加还原，但也会出现局部氧化事件增强的证据。
