Yao Zhao, Zhang Yunlong, Li Hongyan, Deng Zeyuan, Zhang Xiaoping
State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China.
State Key Lab of Food Science and Technology, Nanchang University, Nanchang 330047, China.
J Trace Elem Med Biol. 2015 Jan;29:182-7. doi: 10.1016/j.jtemb.2014.08.004. Epub 2014 Aug 21.
The present study was conduced to investigate the synergistic effects of combined treatments with Se-methylselenocysteine (SeMSC) and vitamin E (Vit E) in reversing oxidative stress induced by ethanol in serum and different tissues of rats. Sixty female rats were randomly divided into six groups for 30 days' consecutive pretreatments as followed: control (I), physiological saline (II), 2.8μgkg(-1) Se as SeMSC (III), 2.8μgkg(-1) Se as sodium selenite (Na2SeO3, IV), 5mgkg(-1) α-tocopherol as α-tocopherol acetate (Vit E, V), 5mgkg(-1) α-tocopherol as α-tocopherol acetate and 2.8μgkg(-1) Se as SeMSC (VI). All animals in groups II-VI were treated by ethanol treatment to cause oxidative stress. After 6h of ethanol treatment, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA), glutathione (GSH) and carbonyl protein (CP) in the serum, liver, heart and kidney were measured. The result showed that the individual SeSMC, Na2SeO3 and vitamin E could effectively increase the SOD, T-AOC, GSH-Px and GSH contents as well as significantly decrease the MDA and CP concentrations in the tissues of ethanol-induced rats. At the same dose on different forms of Se, SeMSC showed greater antioxidant activity than Na2SeO3. Moreover, group VI (SeMSC and α-tocopherol acetate) showed much better antioxidant activity than individual group III (SeMSC) and V (α-tocopherol acetate) due to the synergistic effect.
本研究旨在探讨硒代甲基硒代半胱氨酸（SeMSC）和维生素E（Vit E）联合治疗对逆转乙醇诱导的大鼠血清和不同组织氧化应激的协同作用。将60只雌性大鼠随机分为六组，连续预处理30天，分组如下：对照组（I）、生理盐水组（II）、以SeMSC形式给予2.8μg/kg硒（III）、以亚硒酸钠（Na2SeO3）形式给予2.8μg/kg硒（IV）、以α-生育酚醋酸酯形式给予5mg/kgα-生育酚（Vit E，V）、以α-生育酚醋酸酯形式给予5mg/kgα-生育酚并以SeMSC形式给予2.8μg/kg硒（VI）。II-VI组的所有动物均接受乙醇处理以诱导氧化应激。乙醇处理6小时后，测定血清、肝脏、心脏和肾脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活性、总抗氧化能力（T-AOC）、丙二醛（MDA）、谷胱甘肽（GSH）和羰基蛋白（CP）的含量。结果表明，单独的SeMSC、Na2SeO3和维生素E可有效提高乙醇诱导的大鼠组织中SOD、T-AOC、GSH-Px和GSH的含量，并显著降低MDA和CP的浓度。在相同剂量的不同形式的硒中，SeMSC显示出比Na2SeO3更强的抗氧化活性。此外，由于协同作用，VI组（SeMSC和α-生育酚醋酸酯）显示出比单独的III组（SeMSC）和V组（α-生育酚醋酸酯）更好的抗氧化活性。
