Mukherjee P S, Karnes H T
Department of Pharmacy and Pharmaceutics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0533, USA.
Biomed Chromatogr. 1996 Sep-Oct;10(5):193-204. doi: 10.1002/(SICI)1099-0801(199609)10:5<193::AID-BMC591>3.0.CO;2-B.
Pre- and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, HPLC systems for separation of the conjugates and detection limits of various analytes are presented in this review. High mass sensitivity for various carboxylic acids have been achieved with the hydrazides, anthracenes, and diazoalkanes.
使用合适的发色团或荧光团进行柱前和柱后衍生化常常被用于提高对具有羧基的分析物的检测灵敏度和选择性。紫外和荧光衍生化试剂的主要类别包括香豆素类似物、卤代烃、重氮烷和胺类。本文综述了衍生化反应条件、用于分离缀合物的高效液相色谱系统以及各种分析物的检测限。使用酰肼、蒽和重氮烷已实现了对各种羧酸的高灵敏度检测。
