低浓度的哇巴因刺激神经元中的钠钙交换。
Low concentrations of ouabain stimulate Na/Ca exchange in neurons.
作者信息
Saghian A A, Ayrapetyan S N, Carpenter D O
机构信息
Department of Biophysics, Armenian Academy of Sciences, Yerevan, Armenia.
出版信息
Cell Mol Neurobiol. 1996 Aug;16(4):489-98. doi: 10.1007/BF02150229.
Abstract
- The effects of low concentrations of ouabain on 22Na efflux, 86Rb influx, 45Ca uptake and cyclic AMP levels were studied in snail ganglia. Ouabain, at concentrations below that which inhibits the Na-K pump as monitored by 86Rb influx, activated "reverse mode" Na/Ca exchange, as indicated by an increased 22Na efflux and 45Ca influx. 2. With electrophysiologic recordings ouabain, in the presence of K(+)-free saline to block Na/K transport, caused a membrane hyperpolarization. These concentrations of ouabain also caused elevation of intracellular cyclic AMP levels. 3. We suggest that the ouabain-induced stimulation of Na efflux is due to a stimulation of reverse Na/Ca exchange. since Na/Ca exchange is electrogenic, these observations are most consistent with ouabain stimulation of Na/Ca exchange in a reversed direction (intracellular Na for extracellular Ca). 4. The effect on Na/Ca exchange may be secondary to a rise in intracellular cyclic AMP.
摘要
- 研究了低浓度哇巴因对蜗牛神经节中22Na外流、86Rb内流、45Ca摄取和环磷酸腺苷水平的影响。如通过86Rb内流监测到的那样，在低于抑制钠钾泵的浓度下，哇巴因激活了“反向模式”的钠钙交换，这表现为22Na外流和45Ca内流增加。2. 通过电生理记录发现，在无钾盐溶液存在以阻断钠钾转运的情况下，哇巴因会引起膜超极化。这些浓度的哇巴因还会导致细胞内环磷酸腺苷水平升高。3. 我们认为，哇巴因诱导的钠外流刺激是由于反向钠钙交换受到刺激。由于钠钙交换是生电的，这些观察结果与哇巴因刺激反向（细胞内钠交换细胞外钙）的钠钙交换最为一致。4. 对钠钙交换的影响可能继发于细胞内环磷酸腺苷的升高。
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