Rydholm H, Boström S, Eriksson E, Risberg B
Department of Surgery, Ostra Hospital, Göteborg University, Sweden.
Eur Surg Res. 1996;28(5):380-7. doi: 10.1159/000129480.
Endothelial cells play a central role in fibrinolysis due to their production of both tissue plasminogen activator (t-PA) and plasminogen activator inhibitor type-1 (PAI-1). The purpose of this study was to test the hypothesis that the production of t-PA and PAI-1 from human umbilical vein endothelial cells (HUVEC) and from human adult vein endothelial cells (HAVEC) adopt the same pathways for the regulation of fibrinolysis, and that differences in PAI-1 and t-PA production are only quantitative. HUVEC and HAVEC cultures were exposed to phorbol ester (PMA), endotoxin (LPS) or thrombin, singly or in combination with forskolin or H7. The conditioned medium was collected after 16 h and analyzed for t-PA and PAI-1 antigens. The basal production of both t-PA and PAI-1 was significantly higher from HUVEC than from HAVEC. Exposure to PMA, thrombin or forskolin gave a similar response from the two cell types. In contrast, the results from HUVEC and HAVEC differed significantly, not only in magnitude but also in direction, when the cells were exposed to forskolin coincubated with PMA, LPS or thrombin, and in magnitude alone when exposed to LPS only. The results indicate that there are not only quantitative but also qualitative differences in the production of t-PA and PAI-1 from HUVEC and HAVEC. These differences must be taken into account when data from cells of different origin are compared.
内皮细胞在纤维蛋白溶解过程中起着核心作用，因为它们能产生组织型纤溶酶原激活物（t-PA）和1型纤溶酶原激活物抑制剂（PAI-1）。本研究的目的是检验以下假设：人脐静脉内皮细胞（HUVEC）和人成人静脉内皮细胞（HAVEC）产生t-PA和PAI-1采用相同的纤维蛋白溶解调节途径，并且PAI-1和t-PA产生的差异仅是数量上的。将HUVEC和HAVEC培养物单独或与福斯高林或H7联合暴露于佛波酯（PMA）、内毒素（LPS）或凝血酶。16小时后收集条件培养基并分析t-PA和PAI-1抗原。HUVEC产生的t-PA和PAI-1的基础产量均显著高于HAVEC。暴露于PMA、凝血酶或福斯高林时，两种细胞类型有相似的反应。相反，当细胞暴露于与PMA、LPS或凝血酶共同孵育的福斯高林时，HUVEC和HAVEC的结果不仅在程度上而且在方向上都有显著差异，而仅暴露于LPS时在程度上有差异。结果表明，HUVEC和HAVEC产生t-PA和PAI-1不仅存在数量差异，而且存在质量差异。比较来自不同来源细胞的数据时，必须考虑这些差异。
