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[测定蛋白水解活性的灵敏方法]

[Sensitive method for determination of proteolytic activity].

作者信息

Martinenko F P

出版信息

Ukr Biokhim Zh. 1977 May-Jun;49(3):121-5.

PMID:888221
Abstract

The method is developed for estimating the proteolytic activity on the basis of photometric determination at 350 nm of the complexes of products of enzymic splitting of proteins with chloranyl. The advantages of the method are as follows: its high sensitivity (possibility to determine the amount of the enzymic proteolysis products formed from 2-4 microgram of protein), stability of the complexes (three days), wide ranges for direct dependence of absorption on the proteolysis products concentration (0.08-1.2 scale units), simplicity and possibility to conduct determination in the presence of nucleic, acids components.

摘要

该方法是基于在350nm处通过光度法测定蛋白质酶解产物与氯冉的复合物来估算蛋白水解活性而开发的。该方法的优点如下:灵敏度高(能够测定由2 - 4微克蛋白质形成的酶促蛋白水解产物的量)、复合物稳定性好(三天)、吸收值与蛋白水解产物浓度的直接依赖关系范围广(0.08 - 1.2刻度单位)、操作简单且能够在存在核酸成分的情况下进行测定。

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