Ene-Obong H N, Obizoba I C
Department of Home Science and Nutrition, University of Nigeria, Enugu State, Nigeria.
Plant Foods Hum Nutr. 1995 Dec;48(4):297-309. doi: 10.1007/BF01088489.
The protein quality of Nigerian traditional diets based on the African yambean (AYB) and pigeon pea (PP) were performed by in vivo and in vitro bioassays. The individual foods were processed, cooked, dried and mixed to resemble the traditional diets. Weanling male Wistar rats (45-55 g) were used for the bioassay. The rats were fed ad libitum for 35 days of which 28 days were for growth and 7 days for N-balance. All the eleven diets including casein (control) provided 1.6 g N/100 g diet. The parameters tested include PER, growth, N-balance, BV, NPU and apparent digestibility (AD). The rats fed the AYB diets, except AYB: Agidi (Ag), showed superiority in most of the parameters tested. There were significant positive correlations between PER and NPU (r = 0.925; p < 0.001); PER and AD (r = 0.908; p < 0.001); PER and body weight gain (r = 0.969; p < 0.001). The in vitro protein digestibility of the diets was significantly correlated (r = 0.80; p < 0.01) with the in vivo apparent digestibility. These parameters were used to rank the various diets. Crayfish protein was a better supplement to legume/cereal or legume starchy staple mixtures than leguminous oil seed. However, supplementation of PP: steamed corn (SC) diet with crayfish did not prove beneficial. The result showed that diets based on the African yambean, an under-exploited legume, are nutritious. The need for its reintroduction into the fare of the populace through increased production and appropriate processing technology is stressed. The results of this study can form a base for the standardization of Nigerian diets based on these legumes.
通过体内和体外生物测定法对以非洲木豆(AYB)和木豆(PP)为基础的尼日利亚传统饮食的蛋白质质量进行了评估。将这些单一食物进行加工、烹饪、干燥并混合,以模拟传统饮食。选用断乳雄性Wistar大鼠(45 - 55克)进行生物测定。大鼠自由采食35天,其中28天用于生长,7天用于氮平衡测定。包括酪蛋白(对照)在内的所有11种饮食均提供1.6克氮/100克饮食。所测试的参数包括蛋白质效率比(PER)、生长、氮平衡、生物价(BV)、净蛋白质利用率(NPU)和表观消化率(AD)。除AYB:阿吉迪(Ag)外,喂食AYB饮食的大鼠在大多数测试参数上表现出优势。PER与NPU之间存在显著正相关(r = 0.925;p < 0.001);PER与AD之间(r = 0.908;p < 0.001);PER与体重增加之间(r = 0.969;p < 0.001)。饮食的体外蛋白质消化率与体内表观消化率显著相关(r = 0.80;p < 0.01)。这些参数用于对各种饮食进行排名。小龙虾蛋白比豆科油籽更适合作为豆类/谷物或豆类淀粉主食混合物的补充剂。然而,用小龙虾补充PP:蒸玉米(SC)饮食并未证明有益。结果表明,以非洲木豆这种未充分利用的豆类为基础的饮食具有营养。强调了通过增加产量和适当的加工技术将其重新引入民众饮食的必要性。本研究结果可为基于这些豆类的尼日利亚饮食标准化奠定基础。