Shui Y B, Kojima M, Sasaki K
Department of Ophthalmology, Kanazawa Medical University, Uchinada, Japan.
Ophthalmic Res. 1996;28 Suppl 2:92-101. doi: 10.1159/000267962.
In order to induce experimental steroid cataracts in rat eyes similar morphologically to those seen in human eyes, prednisolone acetate was administered either topically or systemically for 12 months with a low dose of X-irradiation as a cocataractogenic factor. Twenty-seven Brown-Norway rats were randomly divided into a control group (group I) with no steroid administration; an eyedrop group (group II) with a daily 1% prednisolone acetate instillation of a total volume of 1.0 mg/kg in both eyes, and a systemic group (group III) with a daily intramuscular injection of 0.8-1.0 mg/kg prednisolone acetate. The right eyes of animals in each group were X-irradiated with a single dose of 2 Gy. Topical and systemic steroid administrations started 2 weeks after X-irradiation. Anterior segment changes were documented with a slitlamp microscope and an anterior eye segment analysis system once a month. Body weight and blood glucose levels were examined every week and every 2 weeks, respectively. The mortality rates in groups I, II and III were 0, 11 (1/9) and 25% (3/12), respectively. The both lenses in group I showed a gradual increase in light-scattering intensity in the nuclear and supranuclear regions over time. Initial lens changes in both steroid-treated groups were Y-suture dissociation and a slight increase in light-scattering intensity in the posterior supranuclear region 3 months after prednisolone administration. Opacification of the anterior shallow cortex and the posterior subcapsular layer was observed after 10 months. X-irradiated eyes showed more prominent lens opacification as compared with nonirradiated eyes after 10 months in both group II and group III. Either topical or systemic administrations of prednisolone acetate over a long term successfully induced morphological lenticular changes in the rat similar to those found in human steroid-induced cataracts. A low dose of X-irradiation effectively accelerated opacification as a cocataractogenic risk. This new model will allow future investigation of steroid cataracts.
为了在大鼠眼中诱导出形态上与人类眼中所见相似的实验性类固醇性白内障，将醋酸泼尼松龙局部或全身给药12个月，并给予低剂量X射线照射作为并发性白内障形成因素。27只布朗-挪威大鼠被随机分为未给予类固醇的对照组（I组）；双眼每日滴注总量为1.0mg/kg的1%醋酸泼尼松龙滴眼液的滴眼组（II组）；以及每日肌肉注射0.8-1.0mg/kg醋酸泼尼松龙的全身给药组（III组）。每组动物的右眼接受单次2Gy的X射线照射。X射线照射后2周开始局部和全身给予类固醇。每月用裂隙灯显微镜和眼前节分析系统记录眼前节变化。每周和每2周分别检查体重和血糖水平。I组、II组和III组的死亡率分别为0、11%（1/9）和25%（3/12）。I组的两个晶状体随着时间的推移，核区和核上区的光散射强度逐渐增加。两个类固醇治疗组在给予泼尼松龙3个月后，晶状体的初始变化为Y形缝线分离和核后上区光散射强度略有增加。10个月后观察到前浅层皮质和后囊下层浑浊。在II组和III组中，10个月后，与未照射的眼睛相比，接受X射线照射的眼睛晶状体浑浊更明显。长期局部或全身给予醋酸泼尼松龙成功地在大鼠中诱导出与人类类固醇性白内障相似的晶状体形态学变化。低剂量X射线照射作为并发性白内障形成风险有效地加速了晶状体浑浊。这个新模型将有助于未来对类固醇性白内障的研究。
