De Miranda J R, Muñoz M, Wu R, Hull R, Espinoza A M
CIBCM, Universidad de Costa Rica, San José, Costa Rica.
Virus Genes. 1996;12(3):231-7. doi: 10.1007/BF00284643.
The sequence of rice hoja blanca tenuivirus RNA-2 is analysed and compared to its counter-part in rice stripe tenuivirus. The RNA encodes two proteins, in an ambisense arrangement. The 94 kD pc2, located in the complementary sense RNA, has several features typical of viral membrane (glyco)proteins, and also has regions of local homology to the glycoproteins of the Phleboviruses (Bunyaviridae). The 23 kD pv2 lies in the viral sense RNA and has two small conserved domains that are almost exclusively found in retro-viral membrane glycoproteins. Its genome location is analogous to the NSm protein of several of the Bunyaviridae species, which is thought to have a membrane-related function. The two open reading frames are separated by a large intergenic region which, in common with the other tenuivirus ambisense RNA segments, has a short region that is highly conserved between RStV and RHBV. The significance of these results with respect to the virus structure and gene expression is discussed.
对水稻白叶病毒RNA - 2的序列进行了分析，并将其与其在水稻条纹病毒中的对应序列进行了比较。该RNA以双义排列编码两种蛋白质。位于互补义RNA中的94 kD pc2具有病毒膜（糖）蛋白的几个典型特征，并且还与白蛉病毒（布尼亚病毒科）的糖蛋白具有局部同源区域。23 kD pv2位于病毒义RNA中，具有两个几乎仅在逆转录病毒膜糖蛋白中发现的小保守结构域。其基因组位置类似于几种布尼亚病毒科物种的NSm蛋白，被认为具有与膜相关的功能。两个开放阅读框由一个大的基因间隔区隔开，该间隔区与其他纤细病毒双义RNA片段一样，有一个在水稻条纹病毒和水稻白叶病毒之间高度保守的短区域。讨论了这些结果对于病毒结构和基因表达的意义。
