Huiet L, Tsai J H, Falk B W
Department of Plant Pathology, University of California, Davis 95616.
J Gen Virol. 1993 Apr;74 ( Pt 4):549-54. doi: 10.1099/0022-1317-74-4-549.
The entire 1317 nucleotide sequence of the maize stripe virus (MStV) RNA5 was determined. Only one open reading frame (ORF) was identified and was found in the viral complementary RNA (vcRNA). This ORF appears to encode a protein of M(r) 44237, hereafter referred to as NS5 delta. In vitro translation of transcripts representing nearly full-length RNA5 vcRNA yielded products of the predicted size, as well as some smaller, less prominent products. No products were identified from transcripts of viral polarity. RNA hybridization analyses of MStV-infected Zea mays revealed RNAs corresponding only to full-length RNA5, but both positive and negative polarity RNAs were abundant. Analysis of the NS5 amino acid sequence revealed that it is extremely basic, containing 21% arginine and lysine. Database comparisons showed that NS5 had no significant similarity to other protein sequences.
测定了玉米条纹病毒（MStV）RNA5的完整1317个核苷酸序列。仅鉴定出一个开放阅读框（ORF），且该开放阅读框存在于病毒互补RNA（vcRNA）中。此开放阅读框似乎编码一个分子量为44237的蛋白质，以下简称NS5δ。对代表几乎全长RNA5 vcRNA的转录本进行体外翻译，产生了预测大小的产物以及一些较小、不太明显的产物。未从病毒极性的转录本中鉴定出产物。对感染MStV的玉米进行RNA杂交分析，结果显示仅存在与全长RNA5相对应的RNA，但正、负极性RNA均很丰富。对NS5氨基酸序列的分析表明，它具有极强的碱性，含有21%的精氨酸和赖氨酸。数据库比较显示，NS5与其他蛋白质序列无显著相似性。
