番茄3-羟基-3-甲基戊二酰辅酶A还原酶基因2的启动子具有指导高水平表达的异常调控元件。
The promoter for tomato 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 has unusual regulatory elements that direct high-level expression.
作者信息
Daraselia N D, Tarchevskaya S, Narita J O
机构信息
University of Illinois at Chicago, Laboratory for Molecular Biology 60607, USA.
出版信息
Plant Physiol. 1996 Oct;112(2):727-33. doi: 10.1104/pp.112.2.727.
Abstract
The promoter region of tomato (Lycopersicon esculentum) 3-hydroxy-3-methylglutaryl coenzyme A reductase gene 2 (HMG2) has been analyzed using the transient expression of HMG2-luciferase fusions in red fruit pericarp. The mRNA for HMG2 accumulates to high level during fruit ripening, in a pattern that coincides with the synthesis of the carotenoid lycopene. Unlike most promoters, the region that is upstream of the HMG2 TATA element is not required for high-level expression. The 180-bp region containing the TATA element, the 5' untranslated region, and the translation start site are comparable in strength of the full-length 35S cauliflower mosaic virus promoter. Pyrimidine-rich sequences present in the 5' untranslated leader are important in regulating expression. Also, the ATG start region has been found to increase translation efficiency by a factor of 4 to 10. An alternative hairpin secondary structure has been identified surrounding the HMG2 initiator ATG, which could participate in the translational regulation of this locus. HMG2 appears to be a novel class of strong plant promoters that incorporate unusual, positive regulators of gene expression.
摘要
利用番茄红素β-环化酶基因2（HMG2）与荧光素酶的融合基因在红色果实果皮中的瞬时表达，对番茄（Lycopersicon esculentum）3-羟基-3-甲基戊二酰辅酶A还原酶基因2（HMG2）的启动子区域进行了分析。HMG2的mRNA在果实成熟过程中积累到高水平，其模式与类胡萝卜素番茄红素的合成一致。与大多数启动子不同，HMG2 TATA元件上游的区域对于高水平表达不是必需的。包含TATA元件、5'非翻译区和翻译起始位点的180 bp区域，其强度与全长花椰菜花叶病毒35S启动子相当。5'非翻译前导区中富含嘧啶的序列在调节表达中很重要。此外，已发现ATG起始区域可将翻译效率提高4至10倍。在HMG2起始密码子ATG周围鉴定出一种替代的发夹二级结构，它可能参与该基因座的翻译调控。HMG2似乎是一类新型的强植物启动子，包含不同寻常的基因表达正向调节因子。
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