编码小麦3-羟基-3-甲基戊二酰辅酶A还原酶的cDNA的分离与鉴定
Isolation and characterization of cDNAs encoding wheat 3-hydroxy-3-methylglutaryl coenzyme A reductase.
作者信息
Aoyagi K, Beyou A, Moon K, Fang L, Ulrich T
机构信息
Sogetal, Incorporated, Hayward, California 94545.
出版信息
Plant Physiol. 1993 Jun;102(2):623-8. doi: 10.1104/pp.102.2.623.
Abstract
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC 1.1.1.34) is a key enzyme in the isoprenoid biosynthetic pathway. We have isolated partial cDNAs from wheat (Triticum aestivum) using the polymerase chain reaction. Comparison of deduced amino acid sequences of these cDNAs shows that they represent a small family of genes that share a high degree of sequence homology among themselves as well as among genes from other organisms including tomato, Arabidopsis, hamster, human, Drosophila, and yeast. Southern blot analysis reveals the presence of at least four genes. Our results concerning the tissue-specific expression as well as developmental regulation of these HMGR cDNAs highlight the important role of this enzyme in the growth and development of wheat.
摘要
3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR，EC 1.1.1.34）是类异戊二烯生物合成途径中的关键酶。我们利用聚合酶链反应从小麦（普通小麦）中分离出了部分cDNA。对这些cDNA推导的氨基酸序列进行比较表明，它们代表了一个小基因家族，该家族成员之间以及与来自其他生物（包括番茄、拟南芥、仓鼠、人类、果蝇和酵母）的基因之间都具有高度的序列同源性。Southern印迹分析显示至少存在四个基因。我们关于这些HMGR cDNA的组织特异性表达以及发育调控的结果突出了该酶在小麦生长发育中的重要作用。
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