Sugawara T, Matsuda T
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
J Biomed Mater Res. 1996 Oct;32(2):157-64. doi: 10.1002/(SICI)1097-4636(199610)32:2<157::AID-JBM2>3.0.CO;2-S.
Phenylazido-derivatized low-molecular-weight substances and copolymers containing hydrocarbon- or fluorocarbonalkyl group, sulfonato or amino group, and hydroxyl group were prepared. Upon coating and subsequent ultraviolet (UV) light irradiation, covalent fixation of these substances or copolymers took place only at irradiated portions of polymer surfaces, providing a hydrophobic, ionic, or hydrophilic nature. These were verified with electron spectroscopy for chemical analysis (ESCA) spectra and water contact-angle measurement. Endothelial cells adhered on photochemically modified surfaces which have alkyl, sulfonato, or amino groups on their surfaces, whereas little adhesion occurred on a hydroxyl group-bearing hydrogellike surface. When UV light was irradiated through a photomask on the polymer surfaces, patterned and microprocessed surfaces having cell-adhering and nonadhering regions were obtained. The potential application of this photochemical surface processing method in biomedical engineering is discussed.
制备了含有烃基或氟碳烷基、磺酸根基团或氨基以及羟基的苯基叠氮基衍生化的低分子量物质和共聚物。在进行涂覆并随后进行紫外线（UV）照射后，这些物质或共聚物仅在聚合物表面的照射部分发生共价固定，从而呈现出疏水、离子或亲水性。通过化学分析电子能谱（ESCA）光谱和水接触角测量对这些性质进行了验证。内皮细胞粘附在表面具有烷基、磺酸根基团或氨基的光化学修饰表面上，而在带有羟基的水凝胶状表面上几乎没有粘附现象。当通过光掩模对聚合物表面照射紫外线时，可获得具有细胞粘附和非粘附区域的图案化和微加工表面。讨论了这种光化学表面处理方法在生物医学工程中的潜在应用。
