Stockelberg D, Hou M, Rydberg L, Kutti J, Wadenvik H
Department of Medicine, Sahlgrenska University Hospital, University of Göteborg, Sweden.
Transfus Med. 1996 Sep;6(3):243-8. doi: 10.1111/j.1365-3148.1996.tb00075.x.
Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.
已知血型ABO抗原由几种血小板糖蛋白（GP）携带，例如糖蛋白Ib、糖蛋白IIa、糖蛋白IIb、糖蛋白IIa和血小板内皮细胞黏附分子。除了这些蛋白质外，我们最近观察到血型A抗原也表达于一些其他未鉴定的血小板蛋白质（70 - 90 kDa）上，这些蛋白质的电泳迁移率与糖蛋白IV和糖蛋白V非常相似。这些发现促使我们进一步对这些表达ABO的血小板蛋白质进行表征。通过抗原捕获酶联免疫吸附测定（ELISA），其中使用单克隆抗体（mAb）CLB-IVC7和CLB-SWI6来固定相应的抗原糖蛋白IV和糖蛋白V，人抗A特异性结合源自A1血小板的这些蛋白质；源自A2、B或O血小板的糖蛋白IV和糖蛋白V均不与抗A结合。在使用免疫沉淀的糖蛋白IV和糖蛋白V作为抗原的蛋白质印迹分析中，抗A单克隆抗体免疫染色从A1血小板沉淀的糖蛋白IV和糖蛋白V，但不从A2和O血小板沉淀的糖蛋白IV和糖蛋白V。这些结果确凿地证明血型A抗原在血小板糖蛋白IV和糖蛋白V上表达。
