Sakurada T, Sugiyama A, Sakurada C, Tanno K, Sakurada S, Kisara K, Hara A, Abiko Y
Department of Biochemistry, Daiichi College of Pharmaceutical Sciences, Fukuoka, Japan.
Neurochem Int. 1996 Sep;29(3):271-8. doi: 10.1016/0197-0186(96)00004-6.
The intrathecal (i.t.) injection of capsaicin (0.1 nmol/mouse) through a lumbar puncture elicited scratching, biting and licking responses. Pretreatment with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) (320 nmol), by i.t. injection, resulted in a significant inhibition of the behavioural response produced by i.t. capsaicin (0.1 nmol/mouse). Similar behavioural responses were induced by i.t. injections of NMDA (0.4 nmol), kainate (0.05 nmol) or AMPA (0.05 nmol), which were all inhibited by co-administration of L-NAME (20-80 nmol). L-Arginine (600 mg/kg, i.p.) but not D-arginine (600 mg/kg, i.p.) reversed the inhibitory effect of L-NAME on capsaicin-, NMDA-, kainate- and AMPA-induced behavioural response. Scratching, biting and licking responses induced by tachykinin receptor agonists, substance P, [Sar9,Met(O2)11]substance P, neurokinin A and neurokinin B were not affected by co-administration of L-NAME (40 and 80 nmol). These results suggest that spinal nitric oxide may play a significant role in mechanisms of the behavioural response to capsaicin, probably through the release of glutamate, but not tachykinins.
通过腰椎穿刺鞘内注射辣椒素（0.1 nmol/小鼠）引发抓挠、啃咬和舔舐反应。鞘内注射一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯（L-NAME，320 nmol）进行预处理，可显著抑制鞘内注射辣椒素（0.1 nmol/小鼠）所产生的行为反应。鞘内注射N-甲基-D-天冬氨酸（NMDA，0.4 nmol）、海人藻酸（0.05 nmol）或α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA，0.05 nmol）也可诱导类似的行为反应，而同时给予L-NAME（20 - 80 nmol）均可抑制这些反应。L-精氨酸（600 mg/kg，腹腔注射）而非D-精氨酸（600 mg/kg，腹腔注射）可逆转L-NAME对辣椒素、NMDA、海人藻酸和AMPA诱导的行为反应的抑制作用。速激肽受体激动剂P物质、[Sar9,Met(O2)11]P物质、神经激肽A和神经激肽B所诱导的抓挠、啃咬和舔舐反应不受同时给予L-NAME（40和80 nmol）的影响。这些结果表明，脊髓一氧化氮可能在对辣椒素的行为反应机制中发挥重要作用，可能是通过谷氨酸的释放，但不是通过速激肽。
