Aabo K, Hansen K B
Acta Pathol Microbiol Scand A. 1979 May;87A(3):173-7. doi: 10.1111/j.1699-0463.1979.tb00039.x.
Circulating cytoplasmatic megakaryocytes, isolated from central venous blood of rabbits by a saponinhaemolysis leuco-concentration technique, were found to have two morphological appearances: a globular and an oblong form, referring to the shape of the cells as a whole as well as to the shape of the nuclei. The nucleus was either centrally or peripherally located. Of the megakaryocytes observed, 40% were cytoplasmatic and 60% naked nuclei. Regressive changes in the residual nucleus was observed, only 1.5% showing karyorrhexis or karyolysis. Megakaryoblasts and promegakaryocytes were never encountered. The average megakarycyte content per ml central venous blood was 3.8 (range: 0-10). A not previously described cytoplasmatic-ligating process, in which the nucleus seemed to move out of the cytoplasm, forming a naked nucleus and leaving the total cytoplasm intact, was observed. We call this phenomenon "nucleus escape". The function and fate of the intravascular megakaryocytes are briefly discussed.
通过皂素溶血白细胞浓缩技术从兔中心静脉血中分离出的循环细胞质巨核细胞，有两种形态：球形和长方形，这既指细胞整体形状，也指细胞核形状。细胞核要么位于中央，要么位于周边。在所观察的巨核细胞中，40%为细胞质型，60%为裸核型。观察到残留细胞核有退行性变化，仅1.5%出现核碎裂或核溶解。从未发现巨核母细胞和早巨核细胞。每毫升中心静脉血中巨核细胞的平均含量为3.8（范围：0 - 10）。观察到一种此前未描述过的细胞质结扎过程，在此过程中细胞核似乎移出细胞质，形成裸核，而整个细胞质保持完整。我们将此现象称为“核逸出”。本文简要讨论了血管内巨核细胞的功能和命运。
