Su L, Morgan P R, Lane E B
Emory University School of Medicine, Division of Oral, Head and Neck Pathology, Decatur, Georgia, USA.
J Oral Pathol Med. 1996 Jul;25(6):293-301. doi: 10.1111/j.1600-0714.1996.tb00265.x.
Specific mRNA and protein for two major keratins, K14 and K19, were investigated in normal, dysplastic and malignant oral epithelia by combined in situ hybridization and immunohistochemistry. In normal epithelia, K14 mRNA and protein were present almost exclusively in the basal layer of non-cornified, and in rete-processes of cornified, sites. Dysplastic epithelium showed irregular extension of the K14 transcript and protein into superficial cells. In squamous cell carcinoma (SCC), K14 transcript was abundant in most samples whilst in one poorly differentiated carcinoma mRNA but no protein was detected. K19 mRNA and its protein were present predominantly in basal cells of noncornified epithelium, whereas in cornified epithelium only mRNA was detected. In dysplasias, K19 transcript was detected in all specimens but its protein was absent in most cases. Even more variations of K19 expression were observed in SSC. These findings indicate differences in the control of expression of K14 and K19 in normal epithelia and show that regulation is further disturbed during dysplastic change and malignancy.
通过原位杂交和免疫组织化学相结合的方法，对正常、发育异常和恶性口腔上皮中两种主要角蛋白K14和K19的特定mRNA和蛋白质进行了研究。在正常上皮中，K14 mRNA和蛋白质几乎仅存在于非角化部位的基底层以及角化部位的棘层。发育异常的上皮显示K14转录本和蛋白质不规则地延伸至表层细胞。在鳞状细胞癌（SCC）中，大多数样本中K14转录本丰富，而在一个低分化癌中，检测到mRNA但未检测到蛋白质。K19 mRNA及其蛋白质主要存在于非角化上皮的基底细胞中，而在角化上皮中仅检测到mRNA。在发育异常中，所有标本均检测到K19转录本，但大多数病例中未检测到其蛋白质。在SSC中观察到K19表达的更多变化。这些发现表明正常上皮中K14和K19表达调控存在差异，并表明在发育异常变化和恶性肿瘤过程中调控进一步受到干扰。
