Takahashi K, MacDonald D G, Kinane D F
Periodontology Unit, Glasgow Dental Hospital and School, Scotland.
J Oral Pathol Med. 1996 Jul;25(6):331-5. doi: 10.1111/j.1600-0714.1996.tb00272.x.
Immunoglobulin (Ig) kappa (kappa) and lambda (lambda) light chain mRNA-expressing cells were investigated by in situ hybridization (ISH) to assess the local humoral immune response in human dental periapical lesions. Twenty-seven biopsy samples (17 periapical granulomas and 10 radicular cysts) were examined. Both types of light chain mRNA-positive cells were detected in formalin-fixed/paraffin-embedded tissue sections in all samples. Plasma cells showed weak to strong cytoplasmic staining with both probes and background staining was negligible. The ISH methodology is specific and sensitive in detecting Ig light chain mRNAs and retains cell morphology well. kappa to lambda ratios showed moderate variability for both granulomas and cysts (mean = 1.66 +/- 0.85 SD, 1.47 +/- 0.51, respectively). There was no significant difference in light chain distribution between granulomas and cysts. Ig kappa and lambda light chain proteins were also studied by immunohistochemistry (IHC) but the results were disappointing due to excessive background staining. This study confirms that Ig is locally produced in periapical lesions and that the ISH method localises Ig light chain-containing cells better than IHC. The wide variability in kappa/lambda ratio may support the concept of non-specific multibacterial infection in these lesions.
通过原位杂交（ISH）研究免疫球蛋白（Ig）κ（kappa）和λ（lambda）轻链mRNA表达细胞，以评估人类牙齿根尖周病变中的局部体液免疫反应。检查了27个活检样本（17个根尖周肉芽肿和10个根囊肿）。在所有样本的福尔马林固定/石蜡包埋组织切片中均检测到两种类型的轻链mRNA阳性细胞。浆细胞用两种探针均显示出弱至强的细胞质染色，背景染色可忽略不计。ISH方法在检测Ig轻链mRNA方面具有特异性和敏感性，并且能很好地保留细胞形态。κ与λ的比值在肉芽肿和囊肿中均显示出中等程度的变异性（分别为平均值 = 1.66 ± 0.85标准差，1.47 ± 0.51）。肉芽肿和囊肿之间的轻链分布没有显著差异。还通过免疫组织化学（IHC）研究了Ig κ和λ轻链蛋白，但由于背景染色过多，结果令人失望。本研究证实Ig在根尖周病变中是局部产生的，并且ISH方法比IHC能更好地定位含Ig轻链的细胞。κ/λ比值的广泛变异性可能支持这些病变中存在非特异性多细菌感染的概念。
