Johnsson C, Tufveson G, Wahlberg J, Hällgren R
Department of Transplantation Surgery, University Hospital, Uppsala, Sweden.
Kidney Int. 1996 Oct;50(4):1224-9. doi: 10.1038/ki.1996.431.
An accumulation of the connective tissue component, hyaluronan (HA), is known to occur in both syngeneic and allogeneic kidney grafts during the early postoperative period. The presence of HA in the interstitial tissue of the grafts is paralleled by an increased water content, suggesting a role for HA in the development of the transplantation edema. In the present work, the kidney content and distribution of HA was studied in a model of warm renal ischemia in the rat to investigate whether renal ischemia is associated with HA accumulation. Seventy-two hours after a period of warm renal ischemia (30 or 60 min) significantly higher amounts of HA were observed in the left kidney that had been exposed to ischemia, than in the right, healthy kidney. The most pronounced increase was found to occur in the cortex (20 to 40 times), a structure where there normally is almost no presence of HA. In addition, there was a correlation between the relative water content of the kidney and the amount of HA possible to extract from the tissue. The renal accumulation of HA and water was prevented by daily intravenous administration of hyaluronidase. We conclude that renal ischemia induces an accumulation of HA that may increase the risk for the development of interstitial edema, a situation that may be circumvented by hyaluronidase treatment.
已知在术后早期，同基因和异基因肾移植中都会出现结缔组织成分透明质酸（HA）的积聚。移植间质组织中HA的存在伴随着含水量的增加，这表明HA在移植水肿的发展中起作用。在本研究中，在大鼠热缺血模型中研究了肾脏中HA的含量和分布，以调查肾缺血是否与HA积聚有关。在经历30或60分钟热缺血72小时后，发现经历缺血的左肾中HA的含量明显高于右侧健康肾脏。最显著的增加发生在皮质（20至40倍），而皮质通常几乎不存在HA。此外，肾脏的相对含水量与可从组织中提取的HA量之间存在相关性。每日静脉注射透明质酸酶可防止HA和水在肾脏中的积聚。我们得出结论，肾缺血会导致HA积聚，这可能会增加间质水肿发生的风险，而透明质酸酶治疗可能会避免这种情况。
