估算聚合酶链反应效率的简单方法。
Simple procedure for estimating the efficiency of PCR.
作者信息
Bourinbaiar A S, Ajuang-Simbiri K
机构信息
Metatron Inc., New York, NY 10003, USA.
出版信息
Mol Biotechnol. 1996 Aug;6(1):87-9. doi: 10.1007/BF02762328.
PMID:8887366
Abstract
The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.
摘要
采用逐步控制的有限稀释法，以携带单拷贝HIV基因组的8E5淋巴细胞作为内部对照，检测PCR驱动的DNA扩增的延伸效率。结果显示，在PCR试剂盒制造商推荐的标准条件下，DNA模板的指数增长约为理论倍增率的三分之一。
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