一株感染获得性免疫缺陷综合征逆转录病毒后存活的克隆化Leu-3-细胞的生物学及生化特性
Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus.
作者信息
Folks T M, Powell D, Lightfoote M, Koenig S, Fauci A S, Benn S, Rabson A, Daugherty D, Gendelman H E, Hoggan M D
出版信息
J Exp Med. 1986 Jul 1;164(1):280-90. doi: 10.1084/jem.164.1.280.
Abstract
Leu-3- cells that survive infection with the acquired immune deficiency syndrome (AIDS) retrovirus can be induced with IUdR to express infectious virus. A cellular clone (8E5), isolated by limiting dilution of a mass culture of survivor cells, was found to contain a single, integrated provirus that was constitutively expressed. Although IUdR treatment of 8E5 cells failed to induce infectious virus, cocultivation with Leu-3+ cells generated the characteristic syncytia associated with acute AIDS retrovirus infection. The single integrated copy of proviral DNA directs the synthesis of all major viral structural proteins except p64, as monitored by immunoblotting. The relationship of the 8E5 clone to viral latency and persistence is discussed.
摘要
在获得性免疫缺陷综合征（艾滋病）逆转录病毒感染后存活下来的Leu-3-细胞，可用碘脱氧尿苷（IUdR）诱导其表达传染性病毒。通过对存活细胞的大规模培养进行有限稀释分离得到的一个细胞克隆（8E5），被发现含有一个组成型表达的单一整合前病毒。虽然用IUdR处理8E5细胞未能诱导出传染性病毒，但与Leu-3+细胞共培养会产生与急性艾滋病逆转录病毒感染相关的特征性多核巨细胞。通过免疫印迹监测发现，前病毒DNA的单一整合拷贝指导合成除p64之外的所有主要病毒结构蛋白。文中讨论了8E5克隆与病毒潜伏和持续存在的关系。
相似文献
1
Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus.一株感染获得性免疫缺陷综合征逆转录病毒后存活的克隆化Leu-3-细胞的生物学及生化特性
J Exp Med. 1986 Jul 1;164(1):280-90. doi: 10.1084/jem.164.1.280.
2
AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen.艾滋病逆转录病毒诱导的细胞病理学：巨细胞形成与CD4抗原的参与。
Science. 1986 May 30;232(4754):1123-7. doi: 10.1126/science.3010463.
3
Induction of HTLV-III/LAV from a nonvirus-producing T-cell line: implications for latency.从一个不产生病毒的T细胞系中诱导出HTLV-III/LAV：对潜伏感染的意义。
Science. 1986 Feb 7;231(4738):600-2. doi: 10.1126/science.3003906.
4
Characterization of a continuous T-cell line susceptible to the cytopathic effects of the acquired immunodeficiency syndrome (AIDS)-associated retrovirus.一种对获得性免疫缺陷综合征（AIDS）相关逆转录病毒的细胞病变效应敏感的连续T细胞系的特性分析。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4539-43. doi: 10.1073/pnas.82.13.4539.
5
Persistent noncytopathic infection of normal human T lymphocytes with AIDS-associated retrovirus.艾滋病相关逆转录病毒对正常人T淋巴细胞的持续非细胞病变感染。
Science. 1985 Sep 27;229(4720):1400-2. doi: 10.1126/science.2994222.
6
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.CD4（T4）抗原是艾滋病逆转录病毒受体的重要组成部分。
Nature. 1984;312(5996):763-7. doi: 10.1038/312763a0.
7
Influence of the human T-lymphotropic virus/lymphadenopathy-associated virus on functions of human lymphocytes: evidence for immunosuppressive effects and polyclonal B-cell activation by banded viral preparations.人类嗜T淋巴细胞病毒/淋巴结病相关病毒对人淋巴细胞功能的影响：带状病毒制剂具有免疫抑制作用和多克隆B细胞激活作用的证据。
Proc Natl Acad Sci U S A. 1985 Dec;82(23):8198-202. doi: 10.1073/pnas.82.23.8198.
8
Differential susceptibility to the acquired immunodeficiency syndrome retrovirus in cloned cells of human leukemic T-cell line Molt-4.人类白血病T细胞系Molt-4克隆细胞对获得性免疫缺陷综合征逆转录病毒的易感性差异
J Virol. 1986 Mar;57(3):1159-62. doi: 10.1128/JVI.57.3.1159-1162.1986.
9
Proviral DNA of a retrovirus, human T-cell leukemia virus, in two patients with AIDS.两名艾滋病患者体内逆转录病毒——人类T细胞白血病病毒的前病毒DNA。
Science. 1983 May 20;220(4599):862-5. doi: 10.1126/science.6601822.
10
High prevalence of antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV) in AIDS and related conditions but not in other disease states.在艾滋病及相关病症中，针对获得性免疫缺陷综合征（艾滋病）相关逆转录病毒（ARV）的抗体具有高流行率，但在其他疾病状态中则不然。
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5535-9. doi: 10.1073/pnas.82.16.5535.
引用本文的文献
1
Modeling simian immunodeficiency virus (SIV) latency in primary rhesus macaque CD4 T cells.在恒河猴原代CD4 T细胞中模拟猴免疫缺陷病毒（SIV）潜伏感染
bioRxiv. 2025 Jul 28:2025.04.22.650029. doi: 10.1101/2025.04.22.650029.
2
Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes.用于表征跨组织和亚型的HIV-1储存库的HIV SMRTcap的开发与验证。
bioRxiv. 2025 May 5:2025.05.01.651657. doi: 10.1101/2025.05.01.651657.
3
Distinct viral reservoirs and immune signatures in individuals on long-term antiretroviral therapy with perinatally acquired HIV-1.接受围产期获得性HIV-1长期抗逆转录病毒治疗的个体中不同的病毒储存库和免疫特征。
Cell Rep Med. 2025 Jun 17;6(6):102150. doi: 10.1016/j.xcrm.2025.102150. Epub 2025 May 29.
4
A rapid and high-yield method for nucleic acid extraction.一种快速且高产的核酸提取方法。
Sci Rep. 2025 Apr 11;15(1):12479. doi: 10.1038/s41598-025-95226-0.
5
HIV-1 Integrase T218I/S Polymorphisms Do Not Reduce HIV-1 Integrase Inhibitors' Phenotypic Susceptibility.HIV-1整合酶T218I/S多态性不会降低HIV-1整合酶抑制剂的表型敏感性。
AIDS Res Hum Retroviruses. 2025 Jan;41(1):43-54. doi: 10.1089/AID.2023.0128. Epub 2024 Aug 20.
6
The timing of HIV-1 infection of cells that persist on therapy is not strongly influenced by replication competency or cellular tropism of the provirus.在接受治疗后仍持续存在的细胞发生HIV-1感染的时间，并不受前病毒的复制能力或细胞嗜性的强烈影响。
PLoS Pathog. 2024 Feb 29;20(2):e1011974. doi: 10.1371/journal.ppat.1011974. eCollection 2024 Feb.
7
γδ T cells mediate robust anti-HIV functions during antiretroviral therapy regardless of immune checkpoint expression.在抗逆转录病毒治疗期间，γδ T细胞介导强大的抗HIV功能，而与免疫检查点表达无关。
Clin Transl Immunology. 2024 Jan 29;13(2):e1486. doi: 10.1002/cti2.1486. eCollection 2024.
8
HIV Reservoir: How to Measure It?HIV储存库：如何对其进行检测？
Curr HIV/AIDS Rep. 2023 Apr;20(2):29-41. doi: 10.1007/s11904-023-00653-1. Epub 2023 Apr 1.
9
Unique Molecular Identifiers and Multiplexing Amplicons Maximize the Utility of Deep Sequencing To Critically Assess Population Diversity in RNA Viruses.独特分子标识符和多重扩增子最大限度地提高了深度测序在批判性评估 RNA 病毒群体多样性方面的效用。
ACS Infect Dis. 2022 Dec 9;8(12):2505-2514. doi: 10.1021/acsinfecdis.2c00319. Epub 2022 Nov 3.
10
Identification of 2-(4-N,N-Dimethylaminophenyl)-5-methyl-1-phenethyl-1H-benzimidazole targeting HIV-1 CA capsid protein and inhibiting HIV-1 replication in cellulo.鉴定靶向 HIV-1 CA 衣壳蛋白的 2-(4-N,N-二甲基氨基苯基)-5-甲基-1-苯乙基-1H-苯并咪唑并抑制细胞内 HIV-1 复制。
BMC Pharmacol Toxicol. 2022 Jun 28;23(1):43. doi: 10.1186/s40360-022-00581-7.
本文引用的文献
1
Selection of a fixative for identifying T cell subsets, B cells, and macrophages in paraffin-embedded mouse spleen.用于鉴定石蜡包埋小鼠脾脏中T细胞亚群、B细胞和巨噬细胞的固定剂的选择
J Immunol Methods. 1983 Dec 16;65(1-2):137-45. doi: 10.1016/0022-1759(83)90310-1.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Studies of the mechanism of induction of infectious murine leukemia virus from AKR mouse embryo cell lines by 5-iododeoxyuridine and 5-bromodeoxyuridine.关于5-碘脱氧尿苷和5-溴脱氧尿苷诱导AKR小鼠胚胎细胞系产生传染性小鼠白血病病毒机制的研究。
Virology. 1973 Jan;51(1):163-73. doi: 10.1016/0042-6822(73)90376-0.
4
Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine.鼠白血病病毒：5-碘脱氧尿苷和5-溴脱氧尿苷在体外的高频激活作用
Science. 1971 Oct 8;174(4005):155-6. doi: 10.1126/science.174.4005.155.
5
Induction of HTLV-III/LAV from a nonvirus-producing T-cell line: implications for latency.从一个不产生病毒的T细胞系中诱导出HTLV-III/LAV：对潜伏感染的意义。
Science. 1986 Feb 7;231(4738):600-2. doi: 10.1126/science.3003906.
6
Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire.来自北美和扎伊尔的艾滋病逆转录病毒分离株的基因组异质性。
Science. 1985 Nov 22;230(4728):949-51. doi: 10.1126/science.2997922.
7
Slow, persistent replication of lentiviruses: role of tissue macrophages and macrophage precursors in bone marrow.慢病毒的缓慢、持续性复制：组织巨噬细胞和骨髓中巨噬细胞前体的作用
Proc Natl Acad Sci U S A. 1985 Oct;82(20):7086-90. doi: 10.1073/pnas.82.20.7086.
8
Persistent noncytopathic infection of normal human T lymphocytes with AIDS-associated retrovirus.艾滋病相关逆转录病毒对正常人T淋巴细胞的持续非细胞病变感染。
Science. 1985 Sep 27;229(4720):1400-2. doi: 10.1126/science.2994222.
9
Transcription of novel open reading frames of AIDS retrovirus during infection of lymphocytes.艾滋病逆转录病毒新开放阅读框在淋巴细胞感染过程中的转录
Science. 1985 Sep 27;229(4720):1388-90. doi: 10.1126/science.2994220.
10
Characterization of a continuous T-cell line susceptible to the cytopathic effects of the acquired immunodeficiency syndrome (AIDS)-associated retrovirus.一种对获得性免疫缺陷综合征（AIDS）相关逆转录病毒的细胞病变效应敏感的连续T细胞系的特性分析。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4539-43. doi: 10.1073/pnas.82.13.4539.
Zotero 插件