Dröge W, Süssmuth W, Franze R
Adv Exp Med Biol. 1979;114:319-25. doi: 10.1007/978-1-4615-9101-6_51.
A significant in vivo activation of cytotoxic T-lymphocytes (CTL) against trinitrophenyl (TNP)-modified autologous cells and of a DNA-synthesis response in the peripheral lymphnodes is observed in cyclophosphamide (CyP) treated mice after skinpainting with trinitrochlorbenzene (TNCB) or after injection of TNP-coupled spleen cells (TNP-Spl) into the footpads. The activation of these responses can be suppressed by the transfer of spleen cells or lymphnode cells from skinpainted normal mice, but not from skinpainted mice that had been pretreated with CyP. Suppressive activity is also induced by injections of TNP-Spl i.p. or trinitrobenzosulfonate (TNBS) i.v. Optimal activation of suppression occurs with 3--4 days. The suppressive activity is antigen-specific at least in respect to its activation. Suppressor cells of this kind also suppress the induction of delayed hypersensitivity (DH) responses and the priming for in vitro secondary responses. However, these two responses are less sensitive to the suppression, and their in vivo activation is accordingly much less restricted with the in vivo activation of DNA-synthesis and primary CTL responses. DH and CMC memory can be activated ty TNCB skinpainting without pretreatment with CyP.
在用三硝基氯苯（TNCB）进行皮肤涂抹后或向脚垫注射三硝基苯偶联脾细胞（TNP-Spl）后，在环磷酰胺（CyP）处理的小鼠中观察到针对三硝基苯基（TNP）修饰的自体细胞的细胞毒性T淋巴细胞（CTL）的显著体内激活以及外周淋巴结中的DNA合成反应。这些反应的激活可被来自皮肤涂抹正常小鼠的脾细胞或淋巴结细胞的转移所抑制，但不能被经CyP预处理的皮肤涂抹小鼠的脾细胞或淋巴结细胞所抑制。通过腹腔注射TNP-Spl或静脉注射三硝基苯磺酸（TNBS）也可诱导抑制活性。在3 - 4天时出现抑制的最佳激活。至少就其激活而言，这种抑制活性是抗原特异性的。这种抑制细胞也抑制迟发型超敏反应（DH）的诱导和体外二次反应的致敏。然而，这两种反应对抑制的敏感性较低，因此它们的体内激活与DNA合成和原发性CTL反应的体内激活相比受到的限制要小得多。不经过CyP预处理，通过TNCB皮肤涂抹可激活DH和CMC记忆。
