Suessmuth W, Droege W
Z Immunitatsforsch Immunobiol. 1978 Sep;154(5):416-32.
Repeated intravenous injections of high doses of trinitrobenzosulfonate (TNBS) or dinitrobenzosulfonate (DNBS) activate suppressor cells which inhibit the in vivo activation of a primary DNA synthesis response against trinitrochlorobenzene (TNCB) and dinitrochlorobenzene (DNCB), respectively, almost completely and the delayed type hypersensitivity (DH) response only partially. When tested on the DNA-synthesis response, the suppressor cells show excellent specificity with little cross reactivity of TNBS (or DNBS) induced suppressor cells for DNCB- (or TNCB-) specific responses. TNBS- and DNBS-specific suppressor activity is found in spleen cells, mesenteric lymph nodes and peripheral lymph nodes. The activation of suppressor cells is resistant to the early effects of adult thymectomy (ATx), but sensitive to pretreatment with cyclophosphamide (CyP). The suppressor cells are not simply haptenated cells. They need several days for their activation and are inactivated by incubation for 30 minutes at 56 degrees C or by 2,000 R irradiation. Attempts to obtain soluble suppressor factors by in vitro incubation or extraction of these suppressor cells failed.
反复静脉注射高剂量的三硝基苯磺酸（TNBS）或二硝基苯磺酸（DNBS）可激活抑制细胞，这些抑制细胞几乎能完全抑制针对三硝基氯苯（TNCB）和二硝基氯苯（DNCB）的原发性DNA合成反应在体内的激活，而对迟发型超敏反应（DH）的抑制则不完全。当检测对DNA合成反应的影响时，抑制细胞表现出极佳的特异性，TNBS（或DNBS）诱导的抑制细胞对DNCB-（或TNCB-）特异性反应几乎没有交叉反应性。在脾细胞、肠系膜淋巴结和外周淋巴结中可发现TNBS和DNBS特异性抑制活性。抑制细胞的激活对成年胸腺切除（ATx）的早期影响具有抗性，但对环磷酰胺（CyP）预处理敏感。抑制细胞并非简单的半抗原化细胞。它们的激活需要数天时间，在56℃孵育30分钟或接受2000伦琴照射后会失活。通过体外孵育或提取这些抑制细胞来获得可溶性抑制因子的尝试均告失败。
