Mellgren K, Skogby M, Järnås A, Friberg L G, Wadenvik H, Mellgren G
Department of Paediatric Surgery, Ostra Hospital, University of Göteborg, Sweden.
Perfusion. 1996 Sep;11(5):383-8. doi: 10.1177/026765919601100505.
Blood platelets are rapidly activated in contact with biomaterials and, therefore, can be used as markers of the biocompatibility of various components in an extracorporeal system. In the present work two different oxygenators, one membrane oxygenator (Avecor) and one hollow-fibre oxygenator ("Lilliput', Dideco) were compared. Complete in vitro extracorporeal membrane oxygenation circuits were perfused with fresh, heparinized human blood for 24 h. Eight experiments were performed with the hollow-fibre oxygenator and five experiments with the membrane oxygenator. Blood gases, electrolytes, glucose and haematocrit were kept within physiological limits. Platelet count, plasma concentration of beta-thromboglobulin, platelet serotonin content, platelet membrane glycoprotein lb and its degradation product glycocalicin, as well as plasma haemoglobin concentration were assayed. As regards most of these variables, significant differences in favour of the hollow-fibre oxygenator were observed.
血小板与生物材料接触后会迅速被激活，因此可作为体外系统中各种组件生物相容性的标志物。在本研究中，对两种不同的氧合器进行了比较，一种是膜式氧合器（Avecor），另一种是中空纤维氧合器（“小人国”，迪德科公司）。完整的体外膜肺氧合回路用新鲜的肝素化人体血液灌注24小时。使用中空纤维氧合器进行了8次实验，使用膜式氧合器进行了5次实验。血气、电解质、葡萄糖和血细胞比容均保持在生理范围内。检测了血小板计数、β-血小板球蛋白的血浆浓度、血小板5-羟色胺含量、血小板膜糖蛋白lb及其降解产物糖萼蛋白，以及血浆血红蛋白浓度。关于这些变量中的大多数，观察到有利于中空纤维氧合器的显著差异。
