通过内反射傅里叶变换红外光谱法测定支撑脂质膜中的分子有序性
Determination of molecular order in supported lipid membranes by internal reflection Fourier transform infrared spectroscopy.
作者信息
Citra M J, Axelsen P H
机构信息
Department of Pharmacology, Johnson Research Foundation for Molecular Biophysics, University of Pennsylvania, Philadelphia, 19104-6084 USA.
出版信息
Biophys J. 1996 Oct;71(4):1796-805. doi: 10.1016/S0006-3495(96)79380-3.
Abstract
When polarized internal reflection infrared spectroscopy is used to determine molecular order in supported lipid membranes, the results are critically dependent on the accuracy of assumptions made about the evanescent electric field amplitudes in the membrane. In this work, we examine several expressions used for calculating evanescent electric field amplitudes in supported lipid monolayers and bilayers, and test their validity by measuring the infrared dichroism of poly-gamma-benzyl-L-glutamate and poly-beta-benzyl-L-aspartate under conditions in which their molecular order is known. Our results indicate that treating such systems as a simple single interface between two semi-infinite bulk phases is more accurate than the commonly employed thin-film approximation. This implies that earlier conclusions about molecular order in supported lipid membranes may require substantial revision.
摘要
当使用偏振内反射红外光谱法来测定支撑脂质膜中的分子有序性时，结果严重依赖于对膜中倏逝电场振幅所做假设的准确性。在这项工作中，我们研究了用于计算支撑脂质单层和双层中倏逝电场振幅的几种表达式，并通过在已知其分子有序性的条件下测量聚-γ-苄基-L-谷氨酸酯和聚-β-苄基-L-天冬氨酸的红外二色性来测试它们的有效性。我们的结果表明，将此类系统视为两个半无限体相之间的简单单界面比常用的薄膜近似更为准确。这意味着关于支撑脂质膜中分子有序性的早期结论可能需要大幅修正。
相似文献
1
Determination of molecular order in supported lipid membranes by internal reflection Fourier transform infrared spectroscopy.
Biophys J. 1996 Oct;71(4):1796-805. doi: 10.1016/S0006-3495(96)79380-3.
2
Alpha-helical hydrophobic polypeptides form proton-selective channels in lipid bilayers.
Biophys J. 1994 May;66(5):1364-79. doi: 10.1016/S0006-3495(94)80927-0.
3
Quantitative orientation measurements in thin lipid films by attenuated total reflection infrared spectroscopy.
Biophys J. 1999 Jan;76(1 Pt 1):539-51. doi: 10.1016/S0006-3495(99)77222-X.
4
Preparation of unidirectional end-grafted alpha-helical polypeptides by solvent quenching.
J Am Chem Soc. 2003 May 28;125(21):6376-7. doi: 10.1021/ja034428k.
5
Orientation of the infrared transition moments for an alpha-helix.
Biophys J. 2000 May;78(5):2499-510. doi: 10.1016/S0006-3495(00)76795-6.
6
The infrared dichroism of transmembrane helical polypeptides.
Biophys J. 1995 Dec;69(6):2770-81. doi: 10.1016/S0006-3495(95)80150-5.
7
Orientational order determination by internal reflection infrared spectroscopy.
Prog Biophys Mol Biol. 1996;66(3):227-53. doi: 10.1016/s0079-6107(97)00007-2.
8
In situ characterization of N-carboxy anhydride polymerization in nanoporous anodic alumina.
J Phys Chem B. 2009 Mar 12;113(10):3179-89. doi: 10.1021/jp809593d.
9
Structural characterization of membrane proteins and peptides by FTIR and ATR-FTIR spectroscopy.
Methods Mol Biol. 2013;974:177-218. doi: 10.1007/978-1-62703-275-9_9.
10
[Study on the pH-sensitive secondary structure of gamma-PGA embedded with magnetite nanoparticles].
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Aug;29(8):2148-51.
引用本文的文献
1
Simple, Direct Routes to Polymer Brush Traps and Nanostructures for Studies of Diffusional Transport in Supported Lipid Bilayers.
Langmuir. 2017 Apr 18;33(15):3672-3679. doi: 10.1021/acs.langmuir.7b00497. Epub 2017 Apr 5.
2
Peptide-induced domain formation in supported lipid bilayers: direct evidence by combined atomic force and polarized total internal reflection fluorescence microscopy.
Biophys J. 2010 Mar 3;98(5):815-23. doi: 10.1016/j.bpj.2009.12.4327.
3
Bioactive and structural metabolites of pseudomonas and burkholderia species causal agents of cultivated mushrooms diseases.
Perspect Medicin Chem. 2008 May 9;2:81-112.
4
Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.
Biophys J. 2009 Mar 4;96(5):1970-84. doi: 10.1016/j.bpj.2008.11.041.
5
Protein folding in membranes: insights from neutron diffraction studies of a membrane beta-sheet oligomer.
Biophys J. 2008 Jan 15;94(2):492-505. doi: 10.1529/biophysj.107.113183. Epub 2007 Sep 14.
6
The lipid dependence of melittin action investigated by dual-color fluorescence burst analysis.
Biophys J. 2007 Jul 1;93(1):154-63. doi: 10.1529/biophysj.107.106005. Epub 2007 Apr 13.
7
Morphological behavior of lipid bilayers induced by melittin near the phase transition temperature.
Biophys J. 2005 Nov;89(5):3214-22. doi: 10.1529/biophysj.105.059311. Epub 2005 Aug 19.
8
Disorder influence on linear dichroism analyses of smectic phases.
Biophys J. 2005 Jul;89(1):563-71. doi: 10.1529/biophysj.104.058842. Epub 2005 Apr 15.
9
Evaluation of the ordering of membranes in multilayer stacks built on an ATR-FTIR germanium crystal with atomic force microscopy: the case of the H(+),K(+)-ATPase-containing gastric tubulovesicle membranes.
Biophys J. 2004 Aug;87(2):1307-15. doi: 10.1529/biophysj.104.041863.
10
Conformation, orientation, and adsorption kinetics of dermaseptin B2 onto synthetic supports at aqueous/solid interface.
Biophys J. 2003 Aug;85(2):1196-206. doi: 10.1016/S0006-3495(03)74555-X.
本文引用的文献
1
New x-ray evidence on the configuration of polypeptide chains.
Nature. 1951 Jun 30;167(4261):1053-4. doi: 10.1038/1671053a0.
2
The structure of the omegaform of poly-Beta-benzyl-L-aspartate.
J Mol Biol. 1962 Aug;5:230-47. doi: 10.1016/s0022-2836(62)80086-2.
3
The infrared dichroism of transmembrane helical polypeptides.
Biophys J. 1995 Dec;69(6):2770-81. doi: 10.1016/S0006-3495(95)80150-5.
4
Lipid membrane fusion induced by the human immunodeficiency virus type 1 gp41 N-terminal extremity is determined by its orientation in the lipid bilayer.
J Virol. 1996 Jan;70(1):298-304. doi: 10.1128/JVI.70.1.298-304.1996.
5
Structure and orientation of the transmembrane domain of glycophorin A in lipid bilayers.
Biochemistry. 1994 May 24;33(20):6334-41. doi: 10.1021/bi00186a037.
6
Structure and topology of the influenza virus fusion peptide in lipid bilayers.
J Biol Chem. 1995 Nov 17;270(46):27606-14. doi: 10.1074/jbc.270.46.27606.
7
The orientation of melittin in lipid membranes. A polarized infrared spectroscopy study.
Biochim Biophys Acta. 1983 Sep 7;733(2):201-9. doi: 10.1016/0005-2736(83)90523-0.
8
Conformational studies of polymers and copolymers of L-aspartate ester. II. Infrared studies and the factors involved in the formation of the omega-helix.
Biopolymers. 1968;6(7):905-15. doi: 10.1002/bip.1968.360060702.
9
Helix sense of poly- -benzyl-L-aspartate.
J Am Chem Soc. 1972 Jan 12;94(1):297-8. doi: 10.1021/ja00756a063.
10
Attenuated total reflectance Fourier transform infrared studies of the interaction of melittin, two fragments of melittin, and delta-hemolysin with phosphatidylcholines.
Biochemistry. 1987 Dec 15;26(25):8151-8. doi: 10.1021/bi00399a020.
Zotero 插件