通过偏振全内反射荧光-原子力显微镜联用技术探测支撑脂质双层膜的膜序和形貌
Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.
作者信息
Oreopoulos John, Yip Christopher M
机构信息
Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada.
出版信息
Biophys J. 2009 Mar 4;96(5):1970-84. doi: 10.1016/j.bpj.2008.11.041.
Abstract
Determining the local structure, dynamics, and conformational requirements for protein-protein and protein-lipid interactions in membranes is critical to understanding biological processes ranging from signaling to the translocating and membranolytic action of antimicrobial peptides. We report here the application of a combined polarized total internal reflection fluorescence microscopy-in situ atomic force microscopy platform. This platform's ability to image membrane orientational order was demonstrated on DOPC/DSPC/cholesterol model membranes containing the fluorescent membrane probe, DiI-C(20) or BODIPY-PC. Spatially resolved order parameters and fluorophore tilt angles extracted from the polarized total internal reflection fluorescence microscopy images were in good agreement with the topographical details resolved by in situ atomic force microscopy, portending use of this technique for high-resolution characterization of membrane domain structures and peptide-membrane interactions.
摘要
确定膜中蛋白质-蛋白质和蛋白质-脂质相互作用的局部结构、动力学和构象要求对于理解从信号传导到抗菌肽的转运和膜溶解作用等生物过程至关重要。我们在此报告了一种组合的偏振全内反射荧光显微镜-原位原子力显微镜平台的应用。该平台对膜取向有序性成像的能力在含有荧光膜探针DiI-C(20)或BODIPY-PC的DOPC/DSPC/胆固醇模型膜上得到了证明。从偏振全内反射荧光显微镜图像中提取的空间分辨有序参数和荧光团倾斜角与原位原子力显微镜分辨的地形细节高度一致，预示着该技术可用于膜域结构和肽-膜相互作用的高分辨率表征。
相似文献
1
Probing membrane order and topography in supported lipid bilayers by combined polarized total internal reflection fluorescence-atomic force microscopy.通过偏振全内反射荧光-原子力显微镜联用技术探测支撑脂质双层膜的膜序和形貌
Biophys J. 2009 Mar 4;96(5):1970-84. doi: 10.1016/j.bpj.2008.11.041.
2
Combinatorial microscopy for the study of protein-membrane interactions in supported lipid bilayers: Order parameter measurements by combined polarized TIRFM/AFM.用于研究支撑脂质双层中蛋白质-膜相互作用的组合显微镜技术：通过偏振全内反射荧光显微镜/原子力显微镜联用进行序参数测量
J Struct Biol. 2009 Oct;168(1):21-36. doi: 10.1016/j.jsb.2009.02.011. Epub 2009 Mar 5.
3
Peptide-induced domain formation in supported lipid bilayers: direct evidence by combined atomic force and polarized total internal reflection fluorescence microscopy.肽诱导支撑脂质双层中的结构域形成：原子力显微镜和偏振全内反射荧光显微镜的直接证据。
Biophys J. 2010 Mar 3;98(5):815-23. doi: 10.1016/j.bpj.2009.12.4327.
4
Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.膜结构域的相关荧光-原子力显微镜：荧光探针的结构决定脂质定位
Biophys J. 2006 Mar 15;90(6):2170-8. doi: 10.1529/biophysj.105.073510. Epub 2005 Dec 16.
5
Cationic peptide-induced remodelling of model membranes: direct visualization by in situ atomic force microscopy.阳离子肽诱导的模型膜重塑：原位原子力显微镜直接可视化
J Struct Biol. 2008 Apr;162(1):121-38. doi: 10.1016/j.jsb.2007.11.003. Epub 2007 Nov 17.
6
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers.支撑脂质双层膜的原子力显微镜成像与力谱分析
J Vis Exp. 2015 Jul 22(101):e52867. doi: 10.3791/52867.
7
Simultaneous in situ total internal reflectance fluorescence/atomic force microscopy studies of DPPC/dPOPC microdomains in supported planar lipid bilayers.支持的平面脂质双层中DPPC/dPOPC微区的同步原位全内反射荧光/原子力显微镜研究。
J Am Chem Soc. 2003 Oct 1;125(39):11838-9. doi: 10.1021/ja0370894.
8
Phase evolution in cholesterol/DPPC monolayers: atomic force microscopy and near field scanning optical microscopy studies.胆固醇/二棕榈酰磷脂酰胆碱单层膜的相演变：原子力显微镜和近场扫描光学显微镜研究
J Microsc. 2002 Feb;205(Pt 2):136-46. doi: 10.1046/j.0022-2720.2001.00982.x.
9
Lipid asymmetry in DLPC/DSPC-supported lipid bilayers: a combined AFM and fluorescence microscopy study.DLPC/DSPC支撑脂质双层中的脂质不对称性：原子力显微镜与荧光显微镜联合研究
Biophys J. 2006 Jan 1;90(1):228-37. doi: 10.1529/biophysj.105.067066. Epub 2005 Oct 7.
10
Enzymatic generation of ceramide induces membrane restructuring: Correlated AFM and fluorescence imaging of supported bilayers.神经酰胺的酶促生成诱导膜重构：支持双层膜的原子力显微镜与荧光相关成像
J Struct Biol. 2009 Oct;168(1):78-89. doi: 10.1016/j.jsb.2009.03.014. Epub 2009 Apr 5.
引用本文的文献
1
Leaflet-Specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers.固体和聚合物支撑脂质双层膜的小叶特异性结构与动力学
Angew Chem Int Ed Engl. 2025 May;64(21):e202423784. doi: 10.1002/anie.202423784. Epub 2025 Apr 7.
2
Cell membrane sample preparation method of combined AFM and dSTORM analysis.用于原子力显微镜（AFM）和直接随机光学重建显微镜（dSTORM）联合分析的细胞膜样品制备方法
Biophys Rep. 2022 Aug 31;8(4):183-192. doi: 10.52601/bpr.2022.220004.
3
Fluorescence strategies for mapping cell membrane dynamics and structures.用于绘制细胞膜动力学和结构的荧光策略。
APL Bioeng. 2020 May 12;4(2):020901. doi: 10.1063/1.5143945. eCollection 2020 Jun.
4
Biophysical approaches for exploring lipopeptide-lipid interactions.生物物理方法探索脂肽-脂质相互作用。
Biochimie. 2020 Mar;170:173-202. doi: 10.1016/j.biochi.2020.01.009. Epub 2020 Jan 21.
5
The marginal cells of the Caenorhabditis elegans pharynx scavenge cholesterol and other hydrophobic small molecules.秀丽隐杆线虫咽的边缘细胞清除胆固醇和其他疏水性小分子。
Nat Commun. 2019 Sep 2;10(1):3938. doi: 10.1038/s41467-019-11908-0.
6
Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.利用偏振激发的全内反射荧光显微镜成像RBL-2H3细胞中以FcεRI为中心的突触内的膜曲率。
J Imaging. 2019 Jul;5(7). doi: 10.3390/jimaging5070063. Epub 2019 Jul 4.
7
Progress in the Correlative Atomic Force Microscopy and Optical Microscopy.相关原子力显微镜与光学显微镜的进展
Sensors (Basel). 2017 Apr 24;17(4):938. doi: 10.3390/s17040938.
8
Polarization-controlled TIRFM with focal drift and spatial field intensity correction.具有焦点漂移和空间场强校正的偏振控制全内反射荧光显微镜
Biophys J. 2014 Mar 4;106(5):1008-19. doi: 10.1016/j.bpj.2013.12.043.
9
The mechanism of membrane disruption by cytotoxic amyloid oligomers formed by prion protein(106-126) is dependent on bilayer composition.由朊病毒蛋白（106-126）形成的细胞毒性淀粉样寡聚物破坏膜的机制依赖于双层组成。
J Biol Chem. 2014 Apr 11;289(15):10419-10430. doi: 10.1074/jbc.M113.515866. Epub 2014 Feb 19.
10
Combined single cell AFM manipulation and TIRFM for probing the molecular stability of multilayer fibrinogen matrices.联合单细胞原子力显微镜操作和全内反射荧光显微镜探测多层纤维蛋白原基质的分子稳定性。
Ultramicroscopy. 2014 Jan;136:211-5. doi: 10.1016/j.ultramic.2013.10.009. Epub 2013 Oct 19.
本文引用的文献
1
Determination of DPH order parameters in unoriented vesicles.无规向脂质体中 DPH 序参量的测定。
J Fluoresc. 1995 Mar;5(1):39-50. doi: 10.1007/BF00718781.
2
Molecular dynamics simulations of DiI-C18(3) in a DPPC lipid bilayer.二碘辛酯（DiI-C18(3)）在二棕榈酰磷脂酰胆碱（DPPC）脂质双层中的分子动力学模拟
Phys Chem Chem Phys. 2008 Jun 28;10(24):3548-60. doi: 10.1039/b716979e. Epub 2008 May 7.
3
Membrane lipids: where they are and how they behave.膜脂：它们的所在位置及行为方式。
Nat Rev Mol Cell Biol. 2008 Feb;9(2):112-24. doi: 10.1038/nrm2330.
4
Label-free coherent anti-stokes Raman scattering imaging of coexisting lipid domains in single bilayers.单双层中共存脂质域的无标记相干反斯托克斯拉曼散射成像
J Phys Chem B. 2008 Feb 14;112(6):1576-9. doi: 10.1021/jp711527v. Epub 2008 Jan 24.
5
Cationic peptide-induced remodelling of model membranes: direct visualization by in situ atomic force microscopy.阳离子肽诱导的模型膜重塑：原位原子力显微镜直接可视化
J Struct Biol. 2008 Apr;162(1):121-38. doi: 10.1016/j.jsb.2007.11.003. Epub 2007 Nov 17.
6
Molecular perspective of antigen-mediated mast cell signaling.抗原介导的肥大细胞信号传导的分子视角。
J Biol Chem. 2008 Mar 14;283(11):7117-27. doi: 10.1074/jbc.M708879200. Epub 2007 Dec 19.
7
Secretory granule behaviour adjacent to the plasma membrane before and during exocytosis: total internal reflection fluorescence microscopy studies.胞吐作用之前及期间紧邻质膜的分泌颗粒行为：全内反射荧光显微镜研究
Acta Physiol (Oxf). 2008 Feb;192(2):303-7. doi: 10.1111/j.1748-1716.2007.01818.x. Epub 2007 Nov 16.
8
Critical fluctuations in domain-forming lipid mixtures.形成结构域的脂质混合物中的临界涨落。
Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17650-5. doi: 10.1073/pnas.0703513104. Epub 2007 Oct 25.
9
Molecular imaging of membrane interfaces reveals mode of beta-glucosidase activation by saposin C.膜界面的分子成像揭示了鞘脂激活蛋白C激活β-葡萄糖苷酶的模式。
Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17394-9. doi: 10.1073/pnas.0704998104. Epub 2007 Oct 22.
10
From small fluctuations to large-scale phase separation: lateral organization in model membranes containing cholesterol.从小波动到大尺度相分离：含胆固醇模型膜中的侧向组织
Semin Cell Dev Biol. 2007 Oct;18(5):573-82. doi: 10.1016/j.semcdb.2007.08.016. Epub 2007 Sep 4.
Zotero 插件